黄芪甲甙对组胺引起的血管内皮细胞单层通透性增高的作用

摘要

Objective:We studied effect of satragaloside Ⅳ on the increase ofendothelial permeability induced by histamine.Methods:The endothelial cells isolated from Bovine aorta were culture on polycarborate microporous filter membrane to develop confluent endothelial monolayer.Fluid filtration coefficient(Kf),filtration volume(Jv)and osmotic reflective coefficient(σ) to protein were measured in the endothelial monolayer treated by 10-4M histamine for 30 min or treated by 10－4M histamine for 30 min preincubated 10-4g.ml-1 satragaloside Ⅳ for 60 min after perfused either Hanks balanced salt solution or Hanks balanced salt solution containing 5 g.L-1 albumin.Results:The findings showed that Kf,Jv were increased after Perfused Hanks balanced salt solution,and Kf,Jv were increased and σ decreased after perfused Hanks balanced salt solution containing 5 g.L-1 albumin in the endothelial monolayer treated by 10-4M histamine.Application of satragaloside Ⅳ in the endothelial monolayer treated with histamine induced reducing of Kf,and Jv after perfused Hanks balanced salt solution,and induced reduce of Kf,and Jv and σ enhance after perfused Hanks balanced salt solution containing 5 g.L-1 albumin,compared with in the endothelial monolayer treated with histamine purely.Conclusions:The results indicated that increases in the endothelial momolayer permeability induced by histamine,and satragaloside Ⅳ can inhibit increases in the endothelial monolayer permeability induced by histamine.It is necessary that the cellular mechanism of permeability response induced by satragaloside Ⅳ remain to be further elucidated.%目的：本实验观察黄芪甲甙对炎症介质组胺引起的血管内皮通透性增加的作用。方法：从新生牛主动脉分离获得单个内皮细胞，培养于微孔滤膜上直至形成致密单层。通过灌流hanks液或含5g.L-1白蛋白的hanks液后，测定经10－4M组胺溶液处理或经10－4M组胺溶液加10－4g.ml-1黄芪甲甙处理的液体滤过系数(Kf)、液体滤过流量(Jv)和蛋白质渗透压反射系数(σ)。结果：内皮单层经组胺处理后，Kf和Jv降低，σ升高，而黄芪甲甙能够抑制因组胺造成的Kf和Jv降低，以及σ升高。结论：表明组胺能够引起血管内皮单层通透性的增加，而黄芪甲甙能够减轻组胺造成的内皮单层通透性的增加；其作用机制需要进一步研究。