益艾康及HAART治疗对HIV感染者辅助受体CCR5/CXCR4表达的影响

摘要

Objective To study the effects of Yiaikang and highly active antiretroviral therapy (HAART) on coreceptor GCR5/CXCR4 expression and to investigate the relationship between the coreceptor expression and HIV disease progression.Methods:Enrolled 65 cases of HIV infected persons and 10 cases of healthy control,T cell surface CCR5/CXCR4 expression levels were measured by flow cytometry.According to therapeutic method HIV infectors were divided into three groups:untreated group,Yiaikang treatment group,Yiaikang with HAART treatment group,coreceptor expression levels of different groups were compared.Results The expression of CCR5 in CD4 + T cell surface of HIV infection group was higher than that of the control group;while HIV infection group coreceptor CXCR4 expression was lower than that of control group.Correlation analysis showed that CCR5 expression correlated negatively with CD4 +T cell count and positively with viral load;CXCR4 expression correlated positively with CD4 + T cell count and negatively with viral load.Comparing results of coreceptor expression levels in HIV different treatment groups showed Yiaikang treatment can partially restore the coreceptor expression levels and HAART can enhance the effect of Yiaikang.Conclusion:Yiaikang therapy can reduce coreceptor CCR5 expression,increase the expression of the CXCR4 coreceptor in HIV infectors,resuhly partially improve the immune injury caused by AIDS virus infection.%目的:探讨益艾康及HAART治疗对艾滋病病毒感染者辅助受体CCR5/CXCR4表达的影响及辅助受体表达与HIV疾病进展的关系.方法:收集65例HIV感染者及10例健康对照抗凝全血,采用流式细胞技术检测各样本T细胞表面CCR5、CXCR4表达水平.HIV感染者按治疗情况分为未治疗组、益艾康治疗组、益艾康合并HAART治疗组3组,比较不同组别间辅助受体表达水平.结果:HIV感染组CD4+T淋巴细胞表面CCR5表达高于对照组,CXCR4表达低于对照组.相关分析显示,CCR5表达与CD4 +T细胞数成负相关,与病毒载量成正相关;CXCR4表达与CD4+T细胞数成正相关,与病毒载量成负相关.HIV感染者不同治疗组辅助受体表达水平结果显示,益艾康治疗可部分恢复辅助受体的表达水平,HAART治疗可增强益艾康疗效.结论:益艾康治疗可降低艾滋病感染者辅助受体CCR5的表达,增加辅助受体CXCR4的表达,可部分改善艾滋病病毒感染后的免疫损伤.