黄酒多酚对糖尿病心肌病大鼠心肌细胞凋亡的影响

摘要

目的:探究黄酒多酚对糖尿痛心肌病大鼠心肌细胞凋亡的影响.方法:将30只雄性SD大鼠随机分为空白对照组(Control组)、糖尿病心肌病组(DCM组)、糖尿病心肌病+黄酒多酚组(DCM+ YWP组)(n=10).采用单次腹腔注射65 mg/kg链脲佐菌素(STZ)构建糖尿病心肌病大鼠模型,对照组采用相同剂量的枸橼酸缓冲液进行单次腹腔注射,DCM+ YWP组建模后用18 mg/kg黄酒多酚灌胃.12周后观察大鼠一般情况,用多普勒心超评价心脏结构和功能,用电镜观察心肌组织超微结构,用ELISA法检测心肌组织炎症指标,用氧化应激指标检测试剂盒检测心肌组织氧化应激水平,用Westen blot检测心肌组织凋亡相关蛋白Bax、Bcl-2和Caspase-3 (cleaved)的表达水平.结果:与DCM组相比,DCM+ YWP组大鼠血糖水平及体重未出现明显变化;心脏超声显示左室舒张末期直径,左室收缩末期直径降低(P＜0.05),而左室缩短率、左心室射血分数、E/A比值以及Ea/Aa比值均升高(P＜0.05);心肌组织肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)以及白介素6(IL-6)水平下降(P＜0.05);心肌组织氧化应激指标丙二醛(MDA)水平下降、超氧化物歧化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-Px)水平上升(P＜0.05);心肌组织Bax、Caspase-3(cleaved)蛋白的表达水平降低(P＜0.05),Bel-2蛋白的表达水平升高(P＜0.05).结论:黄酒多酚能改善糖尿病心肌病大鼠的心脏功能,降低心肌组织炎症因子和氧化应激水平,抑制糖尿病心肌病大鼠心肌细胞凋亡.%Objective:To investigate the effects of yellow wine polyphenols on the apoptosis of cardiomyocytes in diabetic cardiomyopathy rats.Methods:Thirty SD rats were randomly divided into control group (Control),diabetic cardiomyopathy group (DCM) and diabetic cardiomyopathy treated with yellow wine polyphenols group (DCM + YWP).A single intraperitoneal injection of 65 mg/kg streptozotocin (STZ) was utilized to establish a rat model of DCM.The rats in control group were treated with citrate buffer at the same dose of a single intraperitoneal injection.DCM + YWP group were treated with 18 mg/kg Yellow wine polyphenols by ig after modeling.After treated for 12 weeks,the general condition of rats were observed.The cardiac structure and function of the rats were observed by Doppler echocardiography.The ultrastructure of myocardium were observed using electron microscopy.The inflammation index of myocardial tissue was detected by enzyme-linked immunosorbent assay (ELISA).The oxidative stress in myocardial tissues was assessed by oxidative stress detection kits.The expressions of Bax,Bcl-2 and Caspase-3 (cleaved) in myocardial were detected by Western blot.Results:Compared with DCM group,the blood glucose levels and body weight of rats in the DCM + YWP group were not changed significantly.Echocardiography showed that left ventricular end-diastolic diameter,left ventricular end-systolic diameter were decreased (P ＜ 0.05),while fractional shortening and E/A ratio and Ea/Aa ratio were elevated (P ＜ 0.05).The levels of tumor factor-α(TNF-α),interleukin 1β(IL-1β) and interleukin 6(IL-6) in myocardium were decreased (P ＜ 0.05).The levels of oxidative stress malondiadehyde(MDA) were decreased and Superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) were increased in myocardial tissue (P ＜ 0.05).The expression levels of Bax and Caspase-3 (cleaved) protein in myocardium were decreased (P ＜ 0.05),and the expression of Bcl-2 protein was increased (P ＜ 0.05).Conclusion:Yellow wine polyphenols can improve the diabetic cardiomyopathy rat cardiac function,attenuates inflammation and oxidative stress in diabetic rats,inhibit the apoptosis of cardiomyocytes in diabetic cardiomyopathy.