姜黄素对小鼠胚胎心脏发育中Tbx5表达的影响

摘要

目的 探讨组蛋白乙酰化酶p300抑制剂姜黄素对小鼠胚胎心脏早期发育转录因子Tbx5表达的影响.方法 取清洁级健康成年昆明小鼠,雌雄合笼,受孕成功后于妊娠期第7.5天(E7.5)开始腹腔注射姜黄素75 mg·kg-1·d-1,分别在胚胎心脏发育不同时间点(E11.5、E14.5和E17.5)断颈处死孕鼠,取胎鼠心脏,提取RNA,实时荧光定量(RT)-PCR检测Tbx5的表达.同时设立二甲基亚砜(DMSO)组(腹腔注射相同剂量DMSO)和空白对照组(不做任何处理)作为对照.结果 1.E11.5时,姜黄素组、DMSO组和空白对照组Tbx5的表达水平分别为2.06±0.16、1.08±0.13和1.08±0.18,姜黄素组Tbx5表达较空白组升高1.90倍,差异有统计学意义(P＜0.05).2.E14.5时,姜黄素组、DMSO组和空白组Tbx5的表达水平分别为1.21±0.08、0.71±0.04和0.64±0.10,姜黄素组Tbx5表达较空白组升高1.88倍,差异有统计学意义(P＜0.05).3.E17.5时,姜黄素组、DMSO组和空白对照组Tbx5的表达分别为1.09±0.07、0.79±0.06和0.75±0.06,姜黄素组Tbx5表达较空白组升高1.45倍,差异有统计学意义(P＜0.05).结论 姜黄素能透过胎盘,升高小鼠胚胎心脏早期发育转录因子Tbx5的表达.%Objective To explore the effects of curcumin on the expression of Tbx5 during the development of mice embryonic heart.Methods Adult and healthy female Kunming mice of clean grade were mated with male mice.On embryo day 7.5 ( E7.5 ), the pregnant mice were divided into 3 groups:curcumin group ( pregnant mice were given curcumin by intraperitoneal injection at a dose of 75 mg · kg-1 ·d- 1 ) , dimethyl sulfoxide (DMSO) group (pregnant mice were injected with the same volume of DMSO ) and the blank group (without injection).At different times of embryonic heart development ( E11.5, E14.5 and E17.5 ), pregnant mice were sarif by sacrificed and embryonic hearts were collected.Total RNA were extracted from embryonic hearts and real time reverse transcription polymerase chain reaction was performed to detect the expression of Tbx5.Results 1.On E11.5 ,the expression of Tbx5 in the curcumin group, DMSO group and blank group were 2.06 ± 0.16,1.08 ± 0.13 and 1.08 ± 0.18, respectively.The expression of Tbx5 in curcumin group was increased by 1.90 -fold compared with blank group (P ＜0.05).2.On E14.5 ,the expression of Tbx5 in the 3 groups were 1.21 ±0.08,0.71 ±0.04 and 0.64 ±0.10,respectively ,and the expression of Tbx5 in curcumin group was increased by 1.88 -fold compared with blank group (P ＜ 0.05 ).3.On E17.5,the expression of Tbx5 in the 3 groups were 1.09 ± 0.07,0.79 ± 0.06 and 0.75 ± 0.06, respectively, the expression of Tbx5 in curcumin group was increased by 1.45 - fold compared with blank group ( P ＜ 0.05 ).Conclusion Curcumin can permeate the placenta and increase the expression of Tbx5 ,an important cardiac transcription factor during the early development of mouse heart.