Objective To investigate the dynamic change of Galectin - 3 ( Gal - 3) in mice with coxsackie virus B3 (CVB3 ) induced myocarditis and its clinical significance in viral myocarditis (VMC). Methods A total of 58 healthy male Balb/C mice were randomly divided into VMC group(n =50) and control group(ra =8). The mice in VMC group were treated with 0. 1 mL 10"5 L1 CVB3,and 8 cases of infected mice were respectively sacrificed on the 7* day, 10* day, 14* day and 28* day; while the mice in control group were inoculated intraperito-neally with 0.1 mL eagle reagent and sacrificed on the 28* day. The myocardial pathological changes were observed through light microscope and histopathological scores were calculated. In addition, the expressions of Gal - 3 in myocardial and serum were respectively detected by means of real - time quantitative - polymerase chain reaction and enzyme - linked immunosorbent assay, and then linear correlation analysis with myocardial histopathology scores was conducted. Results 1. Compared with control group, the expression of Gal - 3 mRNA in myocardial in VMC group obviously increased on the 7lh day(0. 91 ±0. 64 vs 10.09 ± 2. 78, P <0. 05) , 10lh day (0.91 ±0. 64 vs 12. 49 ±6. 08,P < 0.05) and 14th day(0.91 ±0. 64 vs 2. 86 ±2. 90,P <0.05). 2. The serum concentration of Gal-3 in VMC group obviously increased on the 7* day(9.70±2.39),10thday (8.98 ±3.03) and 14th day (7.73 ±1.88) compared with control group(5.27 ± 1. 11) (P. <0.05). 3. There were significantly positive correlations between the expression of Gal - 3 mRNA in myocardial and serum with myocardial histopathological scores respectively (r =0.77,0.69;P. <0.05). Conclusions Gal -3 could be involved in the pathogenesis and development of VMC and related with the extent of VMC. It might serve as a new detection mark for VMC.%目的 探讨半乳糖凝集素-3(Gal-3)在小鼠柯萨奇病毒B3(CVB3)所致病毒性心肌炎(VMC)中的表达及其临床意义.方法 选取58只健康雄性Balb/C小鼠,随机分为VMC组(n=50)和对照组(n=8).VMC组小鼠腹腔接种半数组织培养感染剂量( TCID50)为10-5 L-1的Nancy株CVB3病毒液0.1mL,分别于接种第7、10、14、28天随机取8只处死；对照组小鼠腹腔接种不含病毒的eagle氏液0.1 mL,于第28天处死.光镜下观察小鼠心肌组织的病理变化并计算心肌病理积分；运用实时荧光定量PCR检测心肌组织中Gal-3 mRNA的表达,应用ELISA法检测外周血Gal-3水平,并与心肌病理积分作直线相关性分析.结果 1.与对照组小鼠比较,VMC组小鼠心肌组织Gal-3 mRNA的表达在第7天(0.91 ±0.64 vs 10.09 ±2.78)、第10天(0.91 ±0.64 vs 12.49 ±6.08)和第14天(0.91 ±0.64vs2.86±2.90)均明显增高(Pa＜0.05).2.与对照组小鼠外周Gal-3水平(5.27±1.11)比较,VMC组小鼠外周血Gal-3水平在第7天(9.70±2.39)、第10天(8.98±3.03)和第14天(7.73±1.88)也均明显增高(Pa＜0.05).3.小鼠心肌组织中Gal-3 mRNA与外周血中Gal-3表达水平均与心肌病理积分呈直线正相关(r=0.77、0.69,Pa＜0.05).结论 Gal-3参与VMC的发生与发展,且与VMC的病情严重程度有关,可作为VMC新的检测指标.
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