Objective To measure the expressions of microRNAs (miRNA) in the peripheral blood in infantile hepatitis syndrome(IHS) caused by human cytomegalovirus (HCMV) and analyze the relationship between miRNAs and the Ganciclovir (GCV) effectiveness.Methods Forty-four cases of IHS caused by HCMV were recruited from Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine as patients group.Patients were further grouped into GCV nonsensitive group(n =20) and GCV sensitive group(n =24) ;15 healthy infants were taken as healthy control group.One mL peripheral blood were collected at 2 days before the GCV treatment and termination of GCV treatment (or 4 weeks after treatment) respectively.miRNAs were extracted by using special kit,which followed by SYBR Green Ⅰ real-time fluorescence quantitative polymerase chain reaction for testing the relative quantitative (RQ) of hsa-miR-92a,hsa-miR-96,hsa-miR-433 and hsa-miR-183.Results The RQ of hsa-miR-92a,hsa-miR-96,hsa-miR-433 and hsa-miR-183 in patients group were significantly higher than those of healthy control group (all P ＜0.05) ; Before GCV therapy,the RQ of hsa-miR-96 in GCV nonsensitive group (1.24 ±0.62) was lower than that of GCV sensitive group (5.43 ± 1.11) (t =-2.41,P =0.02) ; After GCV treatment,the expression of hsa-miR-96 in GCV nonsensitive group (0.70 ±0.06) was lower than that of GCV sensitive group (6.47 ± 1.01) (t' =-5.46,P ＜0.01),while the expression of hsa-miR-183 showed the same tendency (2.97 ± 0.14 vs 9.84 ± 0.95) (t =-2.52,P =0.019); Furthermore,the expression of hsa-miR-183 and hsa-miR-433 were observed to be down-regulated from 9.79 ± 1.31 and 10.90 ± 0.77 to 2.97 ± 0.14 and 6.20 ± 0.92,respectively after therapy in GCV nonsensitive group (t =-3.54,P =0.002 ; t =2.45,P =0.027).However,the level of hsa-miR-183 after therapy (9.84 ± 0.95) was.higher than that before treatment (9.27 ± 0.96) in GCV sensitive group (t =-2.54,P =0.018),while the level of hsa-miR-433 (6.57 ± 0.71) after therapy was lower than that before treatment (9.88 ± 1.32) (t =3.70,P ＜ 0.001).Conclusions The expression levels of hsa-miR-96 in GCV sensitive group were higher than those in GCV nonsensitive group before or after GCV treatment; the level of hsa-miR-183 in GCV sensitive group was also higher than that in GCV nonsentitive group.The miRNAs expression levels have significance for clinical practice.%目的 观察人巨细胞病毒(HCMV)所致肝炎综合征婴儿治疗前后外周血microRNA(miRNA)水平变化,探讨宿主miRNA与更昔洛韦(GCV)疗效的关系及临床意义.方法 收集HCMV感染所致肝炎综合征婴儿44例为病例组,分为GCV不敏感组(20例)和GCV敏感组(24例)；15例同期体检健康婴儿为健康对照组.分别在GCV治疗前2d和停药后(或用药4周末),采集患儿外周静脉血l mL,提取miRNA,采用SYBR Green I实时荧光定量PCR方法检测hsa-miR-92a、hsa-miR-96、hsa-miR-433和hsa-miR-183表达水平,结果 用相对表达量表示.结果病例组has-miR-92a、has-miR-96、has-miR-433和has-miR-183表达水平均高于健康对照组,差异均有统计学意义(P均＜0.05)；治疗前GCV不敏感组has-miR-96表达水平(1.24±0.62)低于GCV敏感组患儿(5.43 ±1.11),差异有统计学意义(t=-2.41,P=0.02)；治疗后GCV不敏感组has-miR-96(0.70±0.06)和has-miR-183 (2.97±0.14)均明显低于敏感组(6.47±1.01、9.84 ±0.95),差异均有统计学意义(t’=-5.46,P＜0.01;t=-2.52,P=0.019)；GCV不敏感组has-miR-183治疗后(2.97±0.14)较治疗前(9.79±1.31)明显下降,has-miR-433治疗后(6.20±0.92)较治疗前(10.90±0.77)表达水平下降,差异均有统计学意义(t=-3.54,P=0.002;t =2.45,P=0.027)；GCV敏感组has-miR-183表达水平治疗后(9.84±0.95)较治疗前(9.27±0.96)升高,has-miR-433治疗后(6.57 ±0.71)较治疗前(9.88±1.32)下降,差异均有统计学意义(t=-2.54,P=0.018;t =3.70,P＜0.001).结论 GCV敏感患儿用药前后has-miR-96表达均高于不敏感患儿,用药后敏感患儿has-miR-183表达也高于不敏感患儿,miRNA对临床治疗婴儿肝炎综合征具有指导意义.
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