Morphine, codeine and 6-acetyl morphine in human blood were detemined via solid-phase extra-ction and gas chromatography/mass spectrometry-selected ion monitoring, with ethyl morphine as internal standard, 3 opiates spiked in blank blood were extracted wtih a solid-phase cartridge. The fluent was evapo-rated to dryness followed by being derivatized with MBTFA, and then it was analyzed directly by GC/MS in selected ion mode. The method was validated in a range of 10～1 000 μg/L with the drug-spiked blood, and the reactor responded linearly over the studied range(R~2＞0.99 for the 3 drugs). The relative standard deviations(RSD) for intra and inter-days are less than 10% and 16% respectively;the relative extraction recoveries are 90%～116%, 90%～110% and 79%～102% sequenfyl for 3 drugs above, and LODs were less than 1, 0.5 and 3 μg/L respectively, which promised the perspective application of the method in detecting trace amounts of opium in human blood.%采用混合型强阳离子固相萃取柱(SPE)对血液中的吗啡、可待因、6-单乙酰吗啡3种鸦片类毒品进行提取,提取液吹干后,再用N-甲基-双三氟乙酰胺(MBTFA)衍生化,进行GC/MS-SIM检测. 以乙基吗啡为内标,3种毒品检测的线性相关系数均＞0.99, 线性范围为10～1 000 μg/L,相对回收率分别为90%～116%、90%～110%、79%～102%;日内和日间相对标准偏差分别小于10%和16%;检测限分别为1、0.5和3 μg/L. 该方法灵敏度高、重现性好、操作简便,可用于鸦片类毒品滥用者或中毒者血液中的毒品及其代谢物的检测.
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