3,5-二碘水杨醛缩邻苯二胺Ni(Ⅱ)配合物的合成及生物活性

摘要

The Schiff base consisted of 3 ,5-diiodo-salicyIalidehyde and o-diaminobenzene( L) and its Ni(Ⅱ) metal complex has been synthesized and characterized by 1H NMR,elemental analysis,UV spectra,IR spectra and molar conductance analysis.The main bond parameters of the complex were calculated by full-optimized molecular geometry using Gaussian03 program.The composition of the complex was determined to be Ni (Ⅱ) L.The binding mode between complex and DNA was investigated by UV spectra,viscosities,and DNA competitive binding with ethidium bromide ( EB).The results showed that the intensity of absorption peaks increased when the complex interacted with ct-DNA,and its constant was Kb = 1.129×105 L/mol.The complex will lead to dramatic decrease of fluorescent intensity of EB-DNA.The relative viscosity of cf-DNA increased with the concentration increase of the complex.All indicated that the complex was intercalated into ct-BNA.In addition,the complex is assayed against M.luteus by the punch method.%合成了3,5-二碘水杨醛缩邻苯二胺席夫碱合镍(Ⅱ)配合物.通过核磁共振、元素分析、紫外光谱、红外光谱及摩尔电导对结构进行了表征,用Gaussian03程序优化计算,确定配合物的结构为Ni(Ⅱ)L.通过紫外光谱、粘度法及与溴化乙锭(EB)的竞争实验,研究了配合物与小牛胸腺DNA(ct-DNA)的作用情况.结果显示,配合物与DNA作用时,紫外吸收发生明显的减色效应,其结合常数为Kb=1.129×105 L/mol;EB-DNA体系的荧光强度随配合物的加入迅速减弱；配合物的加入使ct-DNA的粘度增加.这些结果表明,该化合物以插入式与ct-DNA键合.并用打孔法测试了配合物对藤黄微球菌(M.luteus)的抑制作用.