滇桂艾纳香水溶性多糖BRP-B的分离纯化及止血活性

摘要

Water-soluble polysaccharide B(BRP-B) in Blumea riparia(BI. ) DC. was extracted and purified by using membrane separation, discoloration on diethylaminoethyl (DEAE) -cellulose, and sephadex column chromatography of G10 and G50. Results from high-performance gel permeation chromatography (HPGPC) showed that the obtained BRP-B has a narrow-distributed molecular mass, with the number average molecular mass and mass average molecular mass as 2654 and 2716, respectively. The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6. 25 x 10 -2 g/L had the strongest procoagulant activity, the coagulation time was 248. 52 s, and the inhibition rate of coagulation time was 22.42％. In particularly, BRP-B with concentration of 1. 67 x 10 -2, 1. 97 x 10 -2 and 2. 85 x 10 -2 g/L could significantly enhance the frequency of uterus muscle.%用蒸馏水溶解滇桂艾纳香浸膏,从中提取多糖,通过膜分离、三氯乙酸法脱蛋白、二乙氨基乙基(DEAE)纤维素脱色、SephadexG10、SephadexG50 凝胶柱色谱纯化,获得滇桂艾纳香水溶性多糖(BRP-B).由凝胶渗透色谱(HPGPC)确定 BRP-B 为相对分子质量分布均一的多糖,其数均分子量和质均分子量分别为2654 和 2716 Da.以血浆复钙时间(PRT)为指标,研究 BRP-B 的凝血活性,结果显示 BRP-B 质量浓度为6.25×10-2 g/L时,凝血时间为 248.52 s,凝血时间抑制率达 22.42%.小白鼠离体子宫实验结果显示,BRP-B质量浓度为1.67×10-2、1.97×10-2 及 2.85×10-2 g/L 时能明显增加子宫平滑肌的收缩频率.