(1-(4-Vinylbenzyl)-1H-1 ,2,3-triazol4-yl)methanol( VBTM) monomer was synthesized via click chemistry and RAFT polymerization method was utilized to achieve 1 , 2, 3-triazole-functionalized dibloek copolymer( PS-6-PVBTM). The diblock copolyraer was spin-coated on gold substrate to form a thin film consisted of nano-spherieal structures with enriuhed PS as core and PVBTM as corona. After being annealed at 90 ℃ for 24 h, the spherical structure was changed to donut-like nano-structure with PS enriched on the surface. Adsorption of proteins on the spin-coated thin film was studied by surface plasma reasonance. The film could absorb bovine serum albumin ( BSA) in an acid buffer solution and the absorption capacity was increased as pH decreased while it did not adsorb BSA and myoglobin in a neutral buffer solution. The film adsorbed lysozyme with a pI of 11. 0, and the adsorption capacity was weakened by 0. 26 ng/mm2 after being annealed. The electrostatic interaction was supposed to be the governing role of adsorption.%通过“点击化学”反应合成了含1,2,3-三氮唑官能团的单体(VBTM),再采用可逆加成-断裂链转移(RAFT)活性自由基聚合法,制备了含有1,2,3-三氮唑官能团的聚苯乙烯(PS-b-PVBTM)两嵌段共聚物.将PS-b-PVBTM旋涂于金片表面成膜,形成以聚苯乙烯链段为核、PVBTM链段为冠的胶束结构；此薄膜于90℃ 退火24 h后,球状结构转变为表面富集PS的环状结构.采用表面等离子体谐振仪(SPR)原位检测蛋白质在PS-b-PVBTM上的吸附动力学.结果表明,当缓冲液为中性时,聚合物薄膜对牛血清蛋白(BSA)和肌红蛋白(Myoglobin)没有吸附,而对等电点为11的溶菌酶(Lysozyme)则有吸附；经退火处理后的聚合物薄膜对Lysozyme的吸附量减少了0.26 ng/mm2；当缓冲液为酸性时,该聚合物薄膜对BSA产生吸附,且吸附量随缓冲液pH值的降低而增加.PS-b-PVBTM对以上3种蛋白质的吸附为静电吸附.
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