内蒙和吉林产地马勃的对比分析

摘要

To identify and analyze puffballs from Inner Mongolia and Jilin in situ, the SEM, FTIR and pyrolysis gas chromatography mass spectrometry(Py-GCMS) were used for the analysis of the microstructure, elemental composition, main category and chemical composition of small molecules, etc. The SEM results indicated that puffball from Inner Mongolia was Mycenastrum corium(gaers. ) Desv but from Jilin was Bovistella radicata( Mont. ) Pat. The FTIR spectra showed that the absorptions of amide I bands of proteins of puffball from Inner Mongolia and Jilin were at 1636 cm-1 and 1654 cm-1 , respectively. The second derivative FTIR spectrum of puffball from Jilin had stronger absorption at 1625 cm suggesting that it contained more aromatic compounds, which was in accordance with the results of Py-GCMS that the contents of phenol and p-methylphenol from Jilin were apparently higher than those from Inner Mongolia. In addition, the Py-GCMS results showed the differences in the pyrolysis compositions and contents between two places of origin of puffballs.%采用扫描电子显微镜( SEM)、傅里叶变换红外光谱(FTIR)和裂解-气质联用(Py-GCMS)从微观形貌、元素组成、主要类别及小分子的化学组成等方面对产自内蒙和吉林的马勃中药材直接进行原位分析,并对产自两地的马勃进行区分.SEM结果表明,内蒙马勃属于灰包科栓皮马勃属栓皮马勃；吉林马勃属于灰包科静灰球属长根静灰球菌.FTIR光谱显示,内蒙马勃在酰胺Ⅰ带吸收峰1636 cm-1,而吉林马勃的在1654 cm-1.吉林马勃二阶导数红外光谱在1625 cm-1有较强的吸收峰说明吉林马勃含芳香环骨架的化合物含量高于内蒙马勃,与Py-GCMS的结论中吉林马勃苯酚、对甲苯酚含量明显高于内蒙马勃相符.另外,Py-GCMS结果可以分析出两地马勃的裂解成分及含量的差异.