As a new type of protein redox-based post-translational modification acting as a molecular switch for the modulation of protein functions , the protein methionine sulfoxidation not only acts as a biomarker of cellular oxidative stress , but also regulates redox signaling transductions , which is thus closely related to many diseases , especially neurodegenerative diseases .In many organisms , the only molecule known to reduce the methionine sulfoxide is methionine sulfoxide reductases ( Msrs ) , which can repair the oxidation damage and recover the function of protein thus play important roles in cellular redox balance regulations .In this article, we will first discuss the structure and catalytic mechanism of protein methionine sulfoxide(MetO) and Msrs, then review the recently developed fluorescent probes for MetO/Msrs type A(MsrA), and give a brief prospect at the last.%蛋白质蛋氨酸亚砜化是一种重要的氧化还原依赖的蛋白质翻译后修饰,不仅是氧化应激的重要标志物之一,也是一种蛋白质功能调控开关可影响活性氧信号转导,与一系列疾病尤其是神经退行性疾病的发生发展密切相关.在许多生物体中,蛋氨酸亚砜还原酶是目前已经发现的唯一能将蛋白质蛋氨酸亚砜还原为蛋氨酸的物质,可以修复氧化损伤蛋白,恢复蛋白质功能,调控细胞氧还平衡,对相关疾病的治疗具有非常重要的意义.本文重点介绍蛋氨酸亚砜和蛋氨酸亚砜还原酶的结构和催化机理,综述蛋氨酸亚砜和蛋氨酸亚砜还原酶荧光探针的部分研究进展,对该领域的研究前景进行展望.
展开▼
