毛泡桐花黄酮对小鼠脾和胸腺超微结构及疫功能的影响

摘要

To explore the effect of flavonoids from flowers of Paulownia tomentosa on the ultrastructure of spleen and thymus in mice,120 Kunming mice were randomly divided into four groups (n =30,half male and half female).Using daily intragastric administration of flavonoids from Paulownia tomentosa flower according to the weight to establish the experimental animal model:Mice in flavonoid group Ⅰ,flavonoid group Ⅱ and flavonoid group Ⅲ were drenched (once daily for 28 days) with 40,120 and 360 mg · (kg · d)-1 flavonoid,respectively.Mice in control group were gavaged with equal volume PBS containing 0.5 ％ CMC-Na.On the 28th day of the treatment,spleens and thymuses were collected and organ index was calculated.Proliferation and transformation of spleen lymphocytes induced by ConA were determined by MTT method.Phagocytosis of peritoneal macrophages was tested by neutral red test.The ultrastructure of spleen and thymuses were observed through scanning electron microscope.The results showed that different doses of flavonoids from Paulownia tomentosa flowers can significantly improve the thymus and spleen index and lymphocyte proliferation response (P＜0.05) in a dose-dependent manner.Compared with the control group,the number of mature lymphocytes in mice spleen and thymus increased significantly in the three flavonoid-treated groups,especially in high dose group.The results suggest that flavonoids from Paulownia tomentosa flowers affect the ultrastructure of spleen and thymus by increasing immune organ quality,immune organs mature lymphocyte number.In addition,lymphocyte proliferation response,flavonoids from Paulownia tomentosa flowers has a certain regulation effect on the immune function in mice by reinforcing the T lymphocyte proliferation response of spleen and thymus to the cellular immune function.%为研究毛泡桐花黄酮对小鼠脾和胸腺超微结构的影响,将120只昆明种小白鼠随机分为4组:对照组(灌服0.3mL·d-1含0.5％CMC-Na的PBS)、黄酮Ⅰ组[40mg·(kg·d)-1黄酮]、黄酮Ⅱ组[120mg·(kg·d)-1黄酮]和黄酮Ⅲ组[360mg·(kg·d)-1黄酮],每组30只(雌雄各半),采用灌胃法按体重每日给小鼠灌服毛泡桐花黄酮建立实验动物模型,在处理后第28天处死小鼠,采集脾和胸腺,计算脏器指数,MTT法检测刀豆素A(ConA)诱导的小鼠脾淋巴细胞增殖转化、中性红试验测定小鼠腹腔巨噬细胞吞噬功能,并采用扫描电镜观察小鼠脾和胸腺结构.结果表明,不同剂量的毛泡桐花黄酮均能显著提高小鼠胸腺和脾指数及淋巴细胞增殖反应和腹腔巨噬细胞吞噬功能(P＜0.05),且呈现一定的剂量依赖性；毛泡桐花黄酮高、中、低3个剂量组小鼠脾和胸腺中成熟淋巴细胞数量均较对照组增多,特别是高剂量组成熟淋巴细胞数量最多.提示,毛泡桐花黄酮通过增加小鼠免疫器官质量、免疫器官中成熟淋巴细胞数量等影响其超微结构.毛泡桐花黄酮通过提高脾T淋巴细胞增殖反应和腹腔巨噬细胞吞噬能力,对小鼠免疫功能具有一定的调控作用.