天祝白牦牛NGB基因的克隆及生物信息学分析

摘要

In order to elucidate the structures of NGB gene, the nearly complete NGB gene sequence of Tianzhu White yak was amplified and analyzed through PCR, cloning, sequencing and the bioinformatics tools. The results showed that the sequence of NGB gene was 3 840 bp in length, including four exons and three introns. 151 amino acids were encoded by the NGB gene and the cloning existed codon bias phenomenon. Compared with NGB gene and its amino acid sequences in taurine cattle (Bos taurus), there were two nucleotide deletions in intron 3 of NGB gene of Tianzhu White yak and two changes of amino acids occurred at the 83rd (S→P) and the 97th(R→Q) positions, respectively. The results lay a foundation for further studying on the genetic characteristics and physiological mechanisms of NGB.%为探讨天祝白牦牛NGB基因的分子结构特征,通过PCR扩增和测序技术,以及生物信息学分析工具,对NGB基因进行克隆、测序及相关生物信息学分析.结果表明:天祝白牦牛NGB基因长3 840 bp,含有4个外显子和3个内含子,可编码151个氨基酸.氨基酸编码存在明显的密码子偏倚现象.与黄牛相比较,白牦牛NGB基因第3内含子存在2个碱基的缺失,氨基酸序列的第83和第97位分别发生(S→P)和(R→Q)的改变.天祝白牦牛NGB基因的成功克隆为进一步研究牦牛NGB的遗传特性和生理机制奠定了基础.