This experiment was conducted to evaluate the effect of Bacillus supplementation in laying hens diets on performance and nutrient apparent digestibility in the anaphase of laying production. A total of 864, 50 -week -old, healthy Hy-line brown laying hens were selected to carry out metabolize experiment using direct collection feces method. These layers were randomly divided into 4 groups with 8 repeats (3 layers in each repeat), included one control group fed a corn-soybean-cottonseed basal diet without any antibiotics and prebiotics and three experiment groups supplemented 0.01% Bacillus, 0.02% Bacillus and 0.02% compound probiotics, respectively. Pre-feeding period lasted 4 days for achieving the optimum performance. The feces from birds of each repeat were collected continuously for 3 days when birds fully adapted to the diets and showed the best production performance. The utilization efficiency of apparent digestibility of energy (AME), crude protein (CP), dry matter (DM), Ca, P and amino acid in diets supplemented with different probiotics were determined. The results showed that 0.02% Bacillus and 0.02% compound probiotics supplementation could significantly improve laying rate, egg production and feed intake (P<0.01). AME, DM and Ser, Glu, Thr, Gly, Arg, Ala, Pro, Val, Phe, He, Leu, His, Lys, Met, Cys in hens during the late laying period have been improved by 0.02% Bacillus and 0.02% compound probiotics supplementation (P<0.05), significantly. 0.02% compound probiotics supplementation could also significantly improve average egg weight (P<0.05), lower feed conversion efficiency and increase of CP, Ca, P (F<0.05). In conclusion, the both probiotics can significantly improve the performance and nutrient apparent digestibility of hens during the late laying period.%本试验选用864只健康海兰褐蛋鸡,随机分为4个处理,在基础日粮中分别添加0.01％、0.02％芽孢杆菌微生态制剂以及0.02％合生素类复合微生态制剂,研究其对蛋鸡生产性能和饲料营养物质消化率的影响.结果表明:0.01％芽孢杆菌组和0.02％复合组极显著提高蛋鸡的产蛋率、产蛋量和采食量(P＜0.01),显著提高日粮表观代谢能、干物质及相关氨基酸(除天冬氨酸和酪氨酸外)的表观消化率(P＜0.05)；0.02％复合组还显著提高蛋鸡平均蛋重(P＜0.05),降低料蛋比(P＜0.05),提高日粮蛋白质、钙、磷的表观消化率(P＜0.05).添加适量的芽孢杆菌及合生素类复合微生态制剂可以提高产蛋后期蛋鸡生产性能和饲料营养物质表观消化率,但过量使用可能会产生不利影响.
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