The present experiment was conducted to examine the effect of energy intake on ovary development in Pekin ducks during the growing period according to the morphology of ovary,plasma profiles,and gene expression.A total of 240 8-week-old Pekin ducks were randomly assigned to 5 dietary treatments of 10.00,10.65,11.30,11.95,12.60 MJ/kg ME with an average energy intake of 1.63 1.73,1.84,1.94,and 2.05 MJ/d by restricted feeding,respectively,and fed for 12 weeks.In our study,high energy intake led to significant increment in body weight and weight gain of ducks (P＜0.05) and it may be due to more fat deposition at this instance.As energy intake increased from 1.63 to 2.05 MJ/d,skin and abdominal fat increased significantly and it was also accompanied with significant increase in plasma.On the other hand,high energy intake stimulated ovary development.Higher ovary percentage was observed (P＜ 0.05) at 1.94 and 2.05 MJ/d and it was also accompanied with larger follicle size according to histological morphology.Furthermore,ovary development might be controlled by gene regulation and it was supported by significant increase in gene expression of follicle-stimulating hormone receptor (FSHR),estrogen receptor (E2R) and leptin receptor (LEPR) at high energy intake.However,the highest (P＜ 0.05) expression level of the luteinizing hormone receptor (LHR) was 1.84 MJ/d group.In conclusion,the energy intake regulate ovary development by the expression of reproduction-related gene and stimulate more fat depostion.%为研究不同的能量摄入量对北京鸭卵巢发育的影响,试验选取8周龄、平均体重为(2 931±291)g的雌性北京鸭240只,随机分为5个组,每组6个重复,每个重复8只.5组试验日粮的能量水平分别为10.00、10.65、11.30、11.95、12.60 MJ/kg.通过统一控制鸭的饲料采食量,使不同日粮能量组试验鸭的平均日能量摄入水平分别控制在1.63、1.73、1.84、1.94、2.05 MJ/d.20周龄时,每个重复中随机选取1只试验鸭进行屠宰,分别观测其卵巢组织形态、血液生化与激素指标,以及卵巢中相关激素受体的mR.NA转录水平.结果表明:提高能量摄入量可显著降低育成期北京鸭的耗料增重比(F/G) (P＜0.05),显著提高日增重(ADG)(P＜0.05);同时能量摄入量能够显著影响血液生化指标并调节繁殖相关激素在卵巢中的表达量(P＜o.05).以上研究提示,提高育成期北京鸭的能量摄入量能显著提高脂肪沉积能力,影响育成期北京鸭卵巢的发育.
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