本试验旨在研究十二指肠灌注亮氨酸( leucine,Leu)对山羊胰腺外分泌及血浆指标的影响,进而为提高反刍动物小肠淀粉利用率提供理论依据.本研究采用4×4拉丁方试验设计,选用4只周岁龄关中奶山羊,手术安装十二指肠瘘管、总胆管插管,进行十二指肠Leu灌注试验(灌注水平分别为0、3、6、9 g/d),研究Leu不同灌注水平对胰腺外分泌功能、血浆葡萄糖及胰岛素浓度的影响.结果表明:十二指肠灌注Leu,血浆中的葡萄糖和胰岛素含量、胰液分泌量、胰液总蛋白质浓度及合成速率、胰蛋白酶、糜蛋白酶和脂肪酶的分泌均不受影响(P＞0.05).随Leu灌注水平的提高,胰腺α-淀粉酶的分泌浓度差异不显著(P＞0.05),而分泌速率呈先升高后降低再回升的变化规律(P＜0.05).结果提示,Leu可不依赖于胰岛素直接长期调控山羊胰腺α-淀粉酶的分泌.%The experiment was conducted to investigate the effect of duodenal infusion of leucine (Leu) on pancreatic exocrine secretion and plasma indices in goats. Four one-year-old goats [(30.1±1.3) kg] fitted with duodenal fistulas and common bile duct cannulas were used in a 4 x 4 Latin square design. Each goat re ceived four treatments: infusion of 0, 3, 6 and 9 g/d Leu into duodenum, respectively. The results showed that duodenal infusion of Leu didn' t affect concentrations of glucose and insulin in plasma, total pancreatic juice secretion, total protein concentration and production rate in pancreatic juice, and activities of trypsin, chymotrypsin, lipase and pancreatic α-amylase (P>0.05). However, the secretion rate of pancreatic α-am ylase was increased when the infusion level of Leu was 3 or 9 g/d (P <0.05). The results indicate that Leu can directly long-term regulate the secretion of pancreatic a-amylase independent of insulin in goats.
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