关于维生素D生物学效价的评定方法主要分为生物学方法和化学方法,然而至今对其没有统一的结论.研究结果大部分显示维生素D3的效价高于或等于维生素D2的效价,且维生素D3及其代谢产物效价的对比大致为维生素D3≤24,25-二羟基维生素D3[24,25-(OH)2D3]=25,26-二羟基维生素D3[25,26-(OH)2D3]≤25-羟基维生素D3[25-(OH) D3]≤1,25-二羟基维生素D3[1,25-(OH) 2D3].影响维生素D生物学效价的因素有多种,其内在因素主要包括关键酶的活性及其反馈调节机制、活性维生素D的结构与维生素D转运蛋白及维生素D受体的亲和性差异等；外在因素主要有试验动物、评价指标及评价方法、光照、维生素D载体等.本文主要从维生素D生物学效价的评定方法、内在和外在的影响因素方面进行综述,为相关研究提供一定的参考依据.%The methods of assessing vitamin D bioavailability are divided into biological assay and specific chemical methods, but so far the methods are still controversial. Most of the research results showed that the potency of vitamin D3 was higher than or equal to the potency of vitamin D2, and the potency of vitamin D3 active components had such roughly order, vitamin D3 sg 24,25-dihydroxyvitamin D3 [ 24,25 ( OH) 2D3 ] = 25 , 26-dihydroxy vitamin D3[25,26 ( OH)2D3 ] ≤25-hydroxy vitamin D3[25(OH)D3] ≤ 1,25-dihydroxy vitamin D3[l ,25(OH)2D3]. Various factors influencing vitamin D bioavailability include internal factors, such as key enzyme activities and their feedback regulation, and the relationship between the structure of biological active vitamin D and its affinity to vitamin D binding protein or vitamin D receptor, and external factors, such as experimental animals, evaluation indicators, assessing methods, light and vitamin D vehicles. In order to provide references for related studies, assessing methods, internal and external influence factors of vitamin D bioavailability are reviewed in this paper. [Chinese Journal of Animal Nutrition, 2012, 24(11) :2079-2084]
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