The experiment was conducted to investigate the effects of lactobacillin on serum biochemical indices and antioxidant function in broilers. A total of 300 one-day-old broiler chickens were randomly divided into five groups with four replicates per group and 15 chickens per replicate. The chickens in control group (group I ) were fed with a basal diet, those in group II were fed with the basal diet supplemented with antibiotics, and those in the other groups (groups III, IV and V ) were fed with the basal diet supplemented with 50, 100 and 200 mg/kg lactobacillin, respectively. The experiment lasted for 70 days. The growth performance, serum antioxidant indices and biochemical parameters were measured on the 28th day and the 70th day of the experiment, respectively. The results showed as follows: 1) comparing with group I , weight gain of broilers in groups II to V was significantly increased and feed/gain was significantly decreased on the 28 th day (P < 0. 05) , and weight gain of broilers in groups II and IV was significantly increased and feed/gain in groups II to V was significantly decreased on the 70th day (P <0. 05). Feed/gain in group IV was significantly lower than that in the other groups (P <0. 05) , while there were no significant differences in that among groups II , III and V (P >0. 05). 2) There was no significant difference in serum urea nitrogen content of broilers in all groups (P >0. 05). Compared with group I , the serum cholesterol content of 28-day-old broilers and alkaline phosphatase activity of 70-day-old broilers in groups II to V were significantly increased (P <0. 01 or P <0. 05) , and the serum total protein content of 28-day-old broilers in groups E and IV was significantly increased (P <0. 05) , but there were no significant differences in the contents of total cholesterol and total protein in serum of 70-day-old broilers among all groups (P >0. 05). There were no significant differences in above all biochemical indices among groups II to V (P > 0. 05). 3) At 28 days of age, the serum SOD activity of broilers in groups II and IV was significantly higher than that in groups I and V (P <0. 05) , but there was no significant difference compared with group III (P >0. 05) ; the serum T-AOC in groups II to V was significantly higher than that in group I (P<0.01), but there was no significant difference among groups II to V (P >0. 05). At 70 days of age, the serum SOD activity and T-AOC of broilers in groups II to IV were significantly higher than those in group I (P <0. 01 or P <0. 05) , but there was no significant difference among groups E to IV (P>0.05). In conclusion, dietary lactobacillin can improve growth performance and antioxidant function of broilers, and the suitable supplemental level in diets is 100 mg/kg.%本试验旨在探讨乳酸菌素对肉鸡血清生化指标和抗氧化功能的影响.300只1日龄肉鸡随机分为5组,每组4个重复,每个重复15只.Ⅰ组为空白对照组,饲喂基础饲粮；Ⅱ组为抗生素对照组；Ⅲ、Ⅳ、Ⅴ组分别饲喂在基础饲粮中添加50、100、200 mg/kg乳酸菌素的试验饲粮.试验期70 d,分别在肉鸡28日龄和70日龄时采样,计算料重比,并检测各组中血液生化指标和抗氧化功能各项指标.结果表明:1)与Ⅰ组比较,试验1 ～28 dⅡ～Ⅴ组肉鸡增重显著升高(P＜0.05),料重比显著降低(P＜0.05);1～70 dⅡ组和Ⅳ组肉鸡增重显著升高(P＜0.05),Ⅱ～Ⅴ组料重比显著降低(P＜0.05),Ⅳ组最低,显著低于其他各组(P＜0.05),Ⅱ、Ⅲ、Ⅴ组之间差异不显著(P＞0.05).2)各组肉鸡血清尿素氮含量无显著差异(P＞0.05).Ⅱ～Ⅴ组28日龄肉鸡血清总胆固醇含量和70日龄肉鸡血清碱性磷酸酶活性均极显著或显著高于Ⅰ组(P＜0.01或P＜0.05)；Ⅱ组和Ⅳ组28日龄肉鸡血清总蛋白含量均显著高于Ⅰ组(P＜0.05)；各组70日龄肉鸡血清总胆固醇和总蛋白含量均无显著差异(P＞0.05).Ⅱ、Ⅲ、Ⅳ、Ⅴ组之间上述各血清生化指标均差异不显著(P＞0.05).3)28日龄时,Ⅱ组和Ⅳ组肉鸡血清超氧化物歧化酶(SOD)活性均显著高于Ⅰ组和Ⅴ组(P＜0.05),但与Ⅲ组差异不显著(P＞0.05)；Ⅱ～Ⅴ组肉鸡血清总抗氧化能力(T-AOC)均极显著高于Ⅰ组(P＜0.01),但组间差异不显著(P＞0.05).70日龄时,Ⅱ～Ⅳ组肉鸡血清SOD活性和T-AOC均显著或极显著高于Ⅰ组(P＜0.05或P＜0.01),但组间差异不显著(P＞0.05).可见,饲粮中添加乳酸菌素可改善肉鸡生长性能,提高其抗氧化功能,以添加100 mg/kg乳酸菌素效果最佳.
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