Effects of different supplemental levels of Enteromorpha prolifera on laying performance,egg quality,immune function,and microflora in feces of laying hens were studied in the present experiment.A total of ninety-six 42-week-old Highland brown laying hens with similar laying rate were randomly divided into 4 groups with 8 replicates per group and 6 birds per replicate.Hens were fed diets supplemented with 0 (control group),1 ％ (group 1),2 ％ (group 2) and 3 ％ Enteromorpha prolifera meal (group 3).The experiment lasted for 28 days.The results showed as follows:dietary supplementation of Enteromorpha prolifera did not have significant effects on laying rate and average daily feed intake (P ＞ 0.05),but Enteromorpha prolifera significantly increased average egg weight (P ＜ 0.05),and significantly decreased feed:egg (P ＜ 0.05).Shell thickness of group 3 was significantly higher than that of control group.Shell calcium content and yolk color of the groups supplemented with Enteromorpha prolifera were significantly improved compared with control group (P ＜ 0.05),but yolk cholesterol content was significantly reduced (P ＜ 0.05).Hens fed Enteromorpha prolifera-supplemented diets had higher serum antibody titers of Newcastle Disease and lymphocyte transformation rate of blood,and there were significant difference between groups 2 and 3 and control group (P ＜ 0.05).In groups 2 and 3,the number of Escherichia coli in feces was significantly reduced (P ＜ 0.01and P ＜ 0.05),while the number of Bifida bacterium in feces was significantly increased compared with control group (P ＜ 0.05 and P ＜ 0.01) ; in groups 1 and 2,the number of Lactobacillus bacterium was significantly increased compared with control group (P ＜ 0.01 and P ＜ 0.05).In conclusion,the supplementation of Enteromorpha prolifera up to 1％-3％ can improve laying performance,egg quality,immune function and microflora in feces of laying hens in different degrees.%本试验旨在研究浒苔添加水平对蛋鸡产蛋性能、蛋品质、免疫功能及粪便微生物区系的影响.试验选用产蛋率基本一致的42周龄海兰褐产蛋鸡96只,随机分为4组,每组设8个重复,每个重复3只.A、B、C组为试验组,饲喂在基础饲粮中分别添加1％、2％、3％浒苔(干粉)的试验饲粮,D组为对照组,饲喂不加浒苔的基础饲粮.正试期28 d.结果表明:试验期间各组鸡产蛋率及平均日采食量无显著差异(P＞0.05),但添加浒苔各组平均蛋重显著高于对照组(P＜0.05),料蛋比显著低于对照组(P＜0.05).3％组蛋壳厚度显著高于对照组(P＜0.05)；各试验组蛋壳钙含量显著高于对照组(P＜0.05),蛋黄颜色显著好于对照组(P＜0.05),蛋黄中胆固醇含量均显著低于对照组(P＜0.05).各试验组血清新城疫抗体效价和血液淋巴细胞转化率均不同程度高于对照组,第28天2％和3％组与对照组差异显著(P＜0.05).2％和3％组粪便中大肠杆菌数量分别极显著和显著降低(P＜0.01和P＜0.05),双歧杆菌数量分别显著和极显著提高(P＜0.05和P＜0.01),1％和2％组乳酸杆菌数量分别极显著和显著提高(P＜0.01和P＜0.05).由此可知,饲粮中添加1％ ～3％的浒苔干粉对蛋鸡产蛋性能、蛋品质、免疫功能和粪便微生物区系均有一定的改善作用.
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