This experiment was conducted to study the effects of glucose oxidase ( GOD) on growth perform-ance, nutrient digestibility, and intestinal microflora and morphology of the weaned piglets. Three hundred three-line crossbred (Duroc×Landrace×Yorkshire) weaned piglets weighted (8.71±0.13) kg were randomly divided into 3 groups, with each group represented by 5 replicates of 20 piglets per replicate. Test groupⅠwas fed a basal diet as control group, test group Ⅱ was fed the basal diet supplemented with 0.1% of chlortetracy-cline, test groupⅢwas fed the basal diet supplemented with 200 g/t of GOD. The trial lasted for 28 days. The results showed as follows: compared with the test group Ⅰ, the average daily gain ( ADG) of the test groupsⅡ and Ⅲ was significantly increased (P<0.05). Diet digestibility of dry matter and crude protein of test groups Ⅱ and Ⅲ was significantly higher ( P<0.05) . The pH of stomach and duodenal of test groups Ⅱ andⅢ was decreased significantly ( P<0.05) . The duodenal villus height and ratio of villus height to crypt depth of test group Ⅲ were significantly increased ( P<0. 05 ) . The number of E. coli of stomach and ileum of test groups ⅡandⅢwas significantly reduced ( P<0.05) , and the number of lactic acid bacteria was significantly increased ( P<0.05) , but the cecum was not significantly different among groups ( P>0.05) . The experiment indicates that the diet supplemented with 200 g/t GOD can reduce the pH and number of E. coli of stomach and duodenum of piglets, increase number of lactic acid bacteria of stomach and duodenum, and improve duodenal villus height and digestibility of dry matter and crude protein, thereby increase the growth performance of pig-lets.%本试验旨在探讨葡萄糖氧化酶对仔猪生长性能、养分消化率及肠道微生物和形态结构的影响。试验采用体重(8.71±0.13) kg的“杜长大”三元杂交断奶仔猪300头，随机分成Ⅰ、Ⅱ、Ⅲ3个组，每个组5个重复，每个重复20头。试验Ⅰ组为对照组，饲喂基础饲粮；试验Ⅱ组在基础饲粮基础上添加0.1%的金霉素；试验Ⅲ组在基础饲粮基础上添加200 g/t的葡萄糖氧化酶。试验期28 d。结果表明，与试验Ⅰ组相比，试验Ⅱ、Ⅲ组平均日增重显著增加(P<0.05)；试验Ⅱ、Ⅲ组饲粮中的干物质和粗蛋白质的消化率显著升高( P<0.05)；试验Ⅱ、Ⅲ组的胃和十二指肠食糜的pH显著下降( P<0.05)；试验Ⅲ组的十二指肠的绒毛高度以及绒毛高度/隐窝深度显著增加(P<0.05)；试验Ⅱ、Ⅲ组胃和回肠中的大肠杆菌数量显著减少(P<0.05)，乳酸菌数量显著增长(P<0.05)，但盲肠中各组差异不显著(P>0.05)。由此可见，饲粮中添加200 g/t葡萄糖氧化酶可以降低仔猪胃和十二指肠的pH和大肠杆菌的数量，增加乳酸菌的数量，改善十二指肠的绒毛高度，提高干物质和粗蛋白质的消化率，从而提高仔猪的生长性能。
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