抗鸡β干扰素单克隆抗体的制备及初步鉴定

摘要

以鸡β干扰素（IFN-β）的重组蛋白pCold-ChIFN-β免疫8周龄Balb/c小鼠，采用杂交瘤技术制备抗鸡β干扰素单克隆抗体，获得两株抗鸡IFN-β的单克隆抗体。经ELISA鉴定，两株单抗的腹水效价均达到10-3以上；Western blot检测结果证明2株单克隆抗体均能与表达的鸡IFN-β发生特异性反应；其中一株单抗可检测浓度小于15 pg/mL的鸡IFN-β。本研究为检测鸡IFN-β提供了一种重要工具，也为评价鸡用疫苗的免疫效果等奠定了物质基础。%　　Eight-week-old Balb/c mice were immunized with purified recombinant pCold-Ch IFN-β. Spleen cells were collected and fused with myeloma SP2/0 cells. Two hybridomas secreting antibodies against IFN-βwere obtained and their ascitic ELISA titers were up to 103. Western blot analysis showed that both McAbs reacted with recombinant IFN-βprotein. The minimum detection concentration of one McAb was below 15 pg/mL IFN-β. The generation of specific McAbs could provided an important tool for detection of IFN-βand evaluation of poultry vaccines.