流产布鲁氏菌的Cu/Zn超氧化物歧化酶的表达及其特性分析

摘要

本研究体外表达了流产布鲁氏菌的Cu/Zn超氧化物歧化酶（Cu/Zn-SOD）并对表达产物进行了免疫原性分析。扩增编码Cu/Zn-SOD的基因，并将其克隆连接到pET-22b(+)载体中。SDS-PAGE 结果显示诱导细菌的裂解物有重组Cu/Zn-SOD表达，分子量为20.8 kDa。Western blot结果显示重组Cu/Zn-SOD均与鼠、牛的阳性血清反应。由此，说明体外表达的重组Cu/Zn-SOD具有很好的抗原性，为今后疫苗开发及诊断试剂的开发奠定了物质基础。%Immunoreactive cytosolic Cu/Zn superoxide dismutase (Cu/Zn-SOD) of Brucella abortus was expressed and characterized in the present study. The gene encoding Cu/Zn-SOD was synthesized using two-round of PCR procedure. The Cu/Zn-SOD gene was cloned into pET-22b(+) vector for expression. The presence of recombinant Cu/Zn-SOD with a relative molecular weight of 20.8 kDa was demonstrated in the lysates of the induced in SDS-PAGE. The recombinant Cu/Zn-SOD reacted with mouse and bovine antiserum samples in Western blot. The preservation of its immunoreactivity indicates that the recombinant Cu/Zn-SOD might be a good antigen for development of vaccine and diagnostic reagents.