2011~2014年上海市屠宰场上市肉猪主要病毒感染情况调查

摘要

为了解上海市屠宰场上市肉猪主要病毒的感染情况,2011~2014年在上海市5个屠宰场共采集759份血清样品,采用ELISA方法进行了口蹄疫、猪瘟、猪繁殖与呼吸综合征抗体检测;采用荧光PCR方法对720份组织样品进行了口蹄疫病毒、猪繁殖与呼吸综合征病毒、猪瘟病毒、伪狂犬病毒、猪圆环病毒2型、猪流感病毒、猪细小病毒7种病毒检测.血清学检测结果显示,猪繁殖与呼吸综合征、猪瘟、口蹄疫抗体平均阳性率依次为96.6%、72.3%、66.4%.荧光PCR检测结果:口蹄疫病毒、猪瘟病毒、猪繁殖与呼吸综合征病毒、伪狂犬病毒均为阴性,但猪圆环病毒2型、猪细小病毒阳性率较高,阳性率分别是37.9%、8.3%.由此可见,上海市屠宰场上市肉猪重大动物疫病防控已有一定效果,但猪圆环病毒2型和猪细小病毒仍具有较高阳性率,对猪群健康存在一定的危害.%To investigate the prevalence of major swine virus diseases in Shanghai, 759 serum samples and 720 visceral tissue samples were collected from slaughtered pigs in Shanghai from 2011 to 2014 and tested using ELISA and real-time PCR. Serological positive rates were 96.6% for Porcine reproductive and respiratory syndrome virus(PRRSV), 72.3% for Foot and mouth disease virus(FMDV) and 66.4% for Classical swine fever virus(CSFV). The positive rates of tissue samples were 37.9% for Porcine circovirus(PCV-2) and 8.3% for Porcine parvovirus(PPV). No tissue samples were found positive for FMDV, CSFV, HPRRSV and PRV. The overall results indicated improvement in animal health but attention was still needed for PCV-2 and PPV.