趋化因子受体CXCR3与尿液TGF-β1在尿道狭窄术后复发过程中的相关性研究

摘要

Objective To investigate the relationship of concentration of TGF-β1 in the urine and the expression level of chemokine receptor CXCR3 in patients with recurrence urethral stricture.Methods Patients with recurring urethral stricture were chosen in this study. The urine concentration of TGF-β1 were detected using the kit of ELISA.By Q-PCR and flow cytometry, the CXCR3 receptor expression in different patients fibroblasts was detected. And the effects of high concentrations of TGF-β1 on different patients fibroblasts CXCR3 receptor expression was also evaluated.Results A significantly lower level of urine TGF-β1 concentration was measured in the control group than that in recurring urethral stricture group and recurring stricture concomitant bladder fistula group (P<0.05). By Q-PCR and flow cytometry, it demonstrated that the level of CXCR3 receptor expression in different patients' fibroblasts was significant lower than that in control group. And high concentrations of TGF-β1 solution can significantly reduce expression of fibroblasts expression in each groups (P<0.05).Conclusion In the process of recurrence of urethral stricture, the TGF-β1 concentration in urine has a negative relationship with the expression levels of fibroblasts CXCR3 receptor, which eventually leading to scarring of the tissue surrounding the urethra.%目的：探索尿道狭窄复发患者疤痕组织细胞中CXCR3水平与其尿液TGF-β1浓度的关联性。方法选取尿道狭窄复发及尿道狭窄复发合并造瘘患者各50例及正常对照组50例，采用ELISA法定量分析患者尿液中TGF-β1的浓度。同时采用流式细胞仪以及Q-PCR技术测定各组患者尿道组织活检分离培养后的成纤维细胞中CXCR3受体的表达情况。并进一步通过流式细胞仪以及Q-PCR技术探究高浓度的TGF-β1对各组成纤维细胞CXCR3受体表达影响。结果相对于正常人群对照组，尿道狭窄复发患者尿液中TGF-β1浓度显著增高（P＜0.05），同时狭窄复发合并造瘘患者较单纯狭窄复发患者其TGF-β1浓度亦有一定程度的升高。进而在流式细胞仪以及Q-PCR技术检测中也提示相应各组成纤维细胞表面CXCR3受体明显低于正常（P＜0.05）。当高浓度的TGF-β1溶液作用于尿道狭窄疤痕组织中的成纤维细胞时，其细胞表面CXCR3的表达均有显著的下降（P＜0.05）。结论在尿道狭窄复发的进程中，尿液中TGF-β1浓度的变化与成纤维细胞表面CXCR3受体表达水平均有一定的负相关性，两者可能在导致致最终尿道周围组织疤痕增生的过程中起到较为重要的作用。