目的：大鼠单侧隐睾模型后，探讨胶质细胞源性神经营养因子（glial cell derived neurotrophic factor， GDNF）对另侧睾丸组织中超氧化物岐化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、白细胞介素-6（IL-6）及睾丸特异性钙结合蛋白86-IV （testis -specific calcium binding proten，TS- CBP86-IV）的影响和机制。方法选取左侧隐睾造模成功雄性SD大鼠体质量（200±30）g，45只，随机分为，A：隐睾组（n=15）；B：生理盐水药物组（n=15）；C：GDNF组（n=15）；D：另选正常对照组（n=15）。每组根据设定处理方式的不同分别进行处理。采集对侧睾丸并行生化指标（SOD、CAT、MDA及IL-6含量）检测；免疫组织化学检测对侧睾丸细胞凋亡指数（AI）；HE染色观察对侧睾丸组织学形态；定时PCR法检测对侧睾丸组织中TS-CBP86-IV mRNA的表达。结果胶质细胞源性神经营养因子药物组（隐睾+GDNF组）生精细胞凋亡明显减少，SOD活性上升，MDA含量下降，IL-6含量下降，TS- CBP86-IVmRNA基因表达明显增强，其与A组、B组、D组比较，差异均有统计学意义。结论 GDNF对单侧大鼠隐睾后对侧睾丸生精功能具有保护作用，并提高抗氧化酶系统的抗氧化及降低血清炎症因子能力，其机制可能与诱导TS-CBP86-IV基因表达有关。%Objective To investigate the effects of glial cell source derived neurotrophic factor on the levels of testis-specific calciumbinding proten CBP86-IV and SOD, CAT, MDA, IL-6 in the contralateral testis in rats. Methods Total of 45 male mice model with left cryptorchidism were randomly divided into three groups such as group A(cryptorchidism group n=15), group B(NS n=15 ), group C (GDNF n=15 ) and group D(the normal contral group n=15). They were killed and the right testis was took out and then its biochemical index was examined including superoxide dismutase(SOD), the activity of catalase(CAT) and level of malonic diethylaldehyde(MDA), interleukin-6(IL-6); Germ cell apoptosis was detected by immunohistochemistry; The histological changes were observed by microscope. The expression of TS- CBP86-IV was detected by real-time quantitative PCR. Results Compared with those of other group,the percentage of apoptotic germcells and the content of MDA and IL-6 were markedly decreased in erdosteine group,but the levels of SOD and CAT were significantly increased. The mRNA expression of TS- CBP86-IV was also enhanced. Conclusion Glial cell derived neurotrophic factor(C group) shows protective effects on contralateral testis. This effect may be achieved through repressing the generation of oxygen-derived free radicals and high expression of TS- CBP86-IV.
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