目的 建立一种新型C57BL6近交系小鼠前列腺癌种植瘤动物模型,观察其生物学特性,并探讨其应用价值.方法 将40只C57BL6近交系小鼠随机平均分为两组:原位组和皮下组,每组20只.在10倍显微镜辅助下将小鼠前列腺癌块分别植于原位组小鼠前列腺组织内和皮下组小鼠皮下,观察两组小鼠的肿瘤转移情况及生存期,并比较两组结果差异是否具有统计学意义.结果 原位组动物在建模后第13～22天出现一般状态衰竭,下腹可触及包块,解剖见癌组织广泛浸润周围盆腔脏器,发生淋巴结转移(16/20)和肺部转移(12/20);皮下组动物生命晚期未出现远处转移.两种模型荷瘤鼠生存时间相比差异有统计学意义(P<0.01).原位组小鼠(17.0±3.5)d短于皮下组(31.8±4.6)d.结论 小鼠前列腺癌瘤块原位种植瘤模型可较好地模拟人类前列腺癌自然生长状况,适于观察前列腺癌在人体环境条件下的生物学行为特性.小鼠前列腺癌皮下种植瘤模型具有较长的生存期,有望用于前列腺癌的实验治疗研究.%Objective To establish a new orthotopic prostate cancer mouse model and observe its biological characteristics, and explore its application value. Methods Forty C57BL6 syngenic mice were randomly and equally divided into two groups such as the 0.t. (A) group and the subcutaneous (B) group. A slice of mice prostate cancer tissue was orthotopically implanted in prostate tissue of mouse under assistance of 10 times microscope in group A, and a slice of mice prostate cancer tissue was subcutaneously implanted into mice in group B. Mice tumor metastasis and survival were comparatively analyzed between the two groups. Results On the 13th-22nd days after modeling, mice in the o.t. group gradually appeared general state failure, abdominal palpable mass. Anatomy analysis of model showed the invasion of the surrounding pelvic organs. They exhibited pelvic lymph mode metastasis (16/20) and lung metastasis (12/20). No metastasis occurred in the control group except local growth and infiltration. The survival time of the o.t. group (17.0±3.5 days) was markedly shorter than that of s.b. group (31.8±4.6 days). There were significant differences in the rate of metastasis, the maximum diameter and the survival time between two groups. Conclusion The orthotopic prostate cancer mouse model which mimics the natural situation of human prostate cancer is an optimal clinical animal model for metastasis analysis. The subcutaneous model had long survive time for for experimental treatment study of prostate cancer.
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