目的:观察电针对脊髓损伤后神经生长抑制因子Sema3A表达的影响,探讨电针对脊髓损伤修复的可能作用机制.方法:将成年雌性SD大鼠分为正常对照组、模型组和电针组,模型和电针组各分7、14、28d3个时间点,建立成年大鼠第9～10胸椎段脊髓全横断损伤模型.电针组于术后当天进行电针治疗,3个组均分别于术后7、14、28d取材,用免疫组织化学ABC法和图像分析技术检测脊髓内Sema3A的表达.结果:术后7、14d时,与正常对照组相比,模型组脊髓损伤处邻近组织内Sema3A表达增强；与模型组相比,电针组脊髓损伤邻近组织内Sema3A表达显著增加；而28d时,电针组、模型组、对照组的Sema3A表达基本一致.结论:电针早期能促进脊髓损伤后Sema3A的表达,从而增强Sema3A对迷乱性出芽的抑制作用,有利于正确的轴突出芽,这可能是其促进脊髓损伤修复的可能机制之一.%Objective: To explore the effect of electro-acupuncture on the expression of nerve growth inhibiting factor Se-ma3A ( Sema3A) in the injured tissues of the completely transected spinal cord,and its mechanism in the repair of spinal cord injury (SCI). Methods: Forty five adult rats were divided into a normal control group,a model group and an electro-acupuncture group ( EC group). All rats underwent complete transection between segment T9 and T10 of the spinal cord. Electro-acupuncture treatment started on the same day after operation in the EC group. The rats were sacrificed on 7 d, 14 d and 28 d after operation. The expression of Sema3A in the tissues of the injured spinal cord was determined by immunohisto-chemical staining. The immunopositive gray scale of the immunoreactivity was detected with computer image analysis technique. Results: The expression of Sema3A in the injured spinal cord was apparently increased in the model group from 7 d to 14 d after operation,compared with the normal control group. The expression of Sema3A in the injured spinal cord was remarkably increased by electro-acupuncture treatment, compared with the model group. On the 28 d, the expression of se-ma3A in all 3 groups was similar, which was near to the normal level. Conclusion: Electro-acupuncture can significantly improve the expression of Sema3A, which is an inhibitor of axon regeneration after SCI. And this effect may be one of the mechanisms by which electro-acupuncture can promote functional recovery after SCI.
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