Objective:To observe the morphological changes in the development of C57/BL6 mice cerebellum and then inves tigate the possible mechanism in mice cerebellum development. Methods: Cerebellum of embryo (E) 12, 14, 16, 18, 20 day (d), postnatal (P) 1, 3, 7, 14, 21, 28 d and P 2, 3, 6, 15 month (M) mice were harvested. Immunohistochemistry and immunoblot assay were used to systematically observe and quantitatively analyze the morphological changes and caspase 3 ex pression in the developing C57/BL6 mice cerebellum. Results: Immunohistochemistry results showed that the caspase 3 ex pression on E12 d-P21 d was located in the cytoplasm and neucleus of mice cerebellum neurons and the caspase3 expression gradually increased on E12 d-P7 d and decreased on P14 d-P21 d. Immunoblotting assay showed that the caspase 3 expres sion increased on El8 d-P7 d, decreased on P14 d-3month and unchanged under a low level after 3 months. Conclusion: The stratification of mice cerebellum is formed in the embryonic period and then gradually matured after birth, and the cerebellum is quickly developing. In addition, apoptosis exists in the cerebellum development and may play an important role in the moulding cerebellum.%目的:观察C57/BL6小鼠小脑发生发育过程中的凋亡蛋白caspase 3表达变化,探讨小脑发生发育的可能机制.方法:应用免疫组织化学技术、免疫印迹对胚龄(E)12、14、16、18、20 d和生后(P)1、3、7、14、21、28 d的仔鼠及生后2、3、6、15个月的C57/BL6小鼠小脑的形态变化及caspase 3的分布进行系统观察和定量分析.结果:免疫组织化学结果显示,E12 d～P21 d,caspase 3在小鼠小脑中均有阳性表达,主要分布在神经元的细胞质和细胞核中.E12 d～P7d,caspase 3表达逐渐增多,P14 d～P21 d逐渐减少.免疫印迹分析表明,E18 d～3个月,caspase 3蛋白表达先逐渐增加后逐渐减少,P7d达高峰,3个月后维持在较低水平.结论:小鼠小脑的片层化结构主要形成于胚胎期,出生后片层化结构逐渐发育成熟,小脑得以迅速发育长大.此外,在小脑发育中存在着大量的细胞凋亡现象,其可能在小脑的塑形中发挥重要的作用.
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