脊髓损伤后虫草素增强突触分化诱导基因1的表达

摘要

Objective:To observe the effect of cordycepin on synapse differentiation induced gene 1 (SynDIG1) expression and the motor function of the nervous system in spinal cord injury,and to explore the internal mechanism of nerve injury repair.Methods:SD rats were randomly divided into control group,injury group,low and high dose treatment group.The injury and treatment groups were injured by spinal cord hemisection.The control group had no spinal cord injury.The injury and treatment groups were respectively injected with cordycepin and normal saline.At 14,21,28,35 d,the motor neural function was evaluated by inclined plane test and Basso Beattie Bresnahan score (BBB).Immunofluorescence staining and Western blotting were used to observe SynDIG1 expression.Results:Western blotting and immunofluorescence staining results showed that the expression of SynDIG1 in injury and treatment group were found at 14 d after the spinal cord injury,and reached the peak at 28 d after the injury.The expression of SynDIG1 of high-dose group was significantly higher than that of the low-dose group and injury group.BBB and inclined plane test score of high-dose group were significantly higher than that of the low-dose group and injury group.Conclusion:Cordycepin can significantly improve the microenvironment of the injury area,activate the endogenous recovery mechanism of the nervous system and promote the expression of SynDIG1.It is beneficial for the recovery of motor functions of the nervous system.%目的:观察脊髓损伤后虫草素对脊髓组织内突触分化诱导基因1(SynDIG1)表达和神经系统运动功能的影响,探讨神经损伤修复的内在机制.方法:将成年雌性SD大鼠随机分为对照组、损伤组、低剂量和高剂量治疗组,对照组不损伤脊髓,治疗组与损伤组行脊髓半横切损伤,随后治疗组给予虫草素处理,损伤组给予生理盐水处理.术后14、21、28、35 d用BBB和斜板试验评价大鼠肢体运动功能,并用免疫荧光和免疫印迹测定脊髓组织内SynDIG1活性.结果:免疫荧光和免疫印迹结果均表明术后14d损伤组和治疗组均有神经细胞SynDIG1表达,在28 d达到高峰,之后呈高水平表达,且SynDIG1水平在高剂量治疗组明显高于低剂量治疗组和损伤组,BBB和斜板试验评分在高剂量治疗组均明显高于低剂量治疗组和损伤组.结论:虫草素明显改善损伤区域的微环境,激活神经系统的内源性修复机制,促进神经细胞SynDIG1大量表达,有利于神经系统运动功能恢复.