A rapid analyzer for the determination of protein were developed. The effects of experimental parameters, including temperature, time, and foreign substances were investigated. Several milk and dairy products were analyzed using the protein fast analyzer. The experimental results showed that protein reagent can react with protein within 1 min at room temperature. The whole process of protein determination in dairy products only takes 5 - 10 min. The protein fast analyzer was applied to the determination of protein in dairy products, the relative error is lower 5% compared to Kjeldahl method and standard amount method of protein in milk power. The relative standard deviation is lower 1%. The determination results of protein were not affected by foreign substances, such as melamine, urea, glycine, ammonium nitrate and so on.%采用研制的蛋白质快速检测仪,系统地考察了温度、时间和干扰物质等因素对蛋白质测定的影响及检测仪的重复性,并将检测仪应用于新鲜乳、纯牛奶、牛奶饮料(核桃、燕麦、红枣)、牛初乳、奶粉、豆奶粉、豆浆粉和鸡蛋等样品中蛋白质的定量测定.实验结果表明,在17～40℃条件下,蛋白质试剂与蛋白质在1 min内即可完成反应,整个蛋白质含量测定过程仅需5～10 min,测定结果的精密度(RSD)小于1％,与凯式定氮法测定结果和标准物质比较,测定结果的相对误差均小于5％,且不受三聚氰胺、尿素、甘氨酸和硝酸铵等非蛋白氮的干扰,表明此仪器具有较好的准确度和重复性,可应用于乳及乳制品中蛋白质的快速定量测定.
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