在弱酸条件下,以邻苯二胺为功能单体,褪黑素为模板,以电化学聚合物法在铂电极表面合成了性能稳定的褪黑素分子印迹聚合物膜.采用循环伏安法(CV)和差分脉冲法(DPV)对分子印迹传感器的识别性能进行了研究.结果表明:此传感器对褪黑素具有快的响应、良好的选择性和高的灵敏度.以K3Fe(CN)6为电子传递媒介,建立了一种差分脉冲伏安法(DPV)间接检测褪黑素的分析方法.在1×10-10～1×10-8 moL/L范围内,褪黑素的浓度与K3Fe(CN)6的相对峰电流变化呈良好的线性关系；检出限为1×10-11 mol/L(S/N=3).将此传感器应用于复合褪黑素及尿样中褪黑素含量的测定,加标平均回收率大于94％.%A sensitive melatonin sensor based on molecularly imprinted electropolymer of o-phenylenediamine was proposed with melatonin as template molecule and o-phenylenediamine as functional monomers under the condition of weak acidity. The recognition performance of molecular imprinted membrane was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) and an indirect analytical method based on the media of potassium ferricyanide (K3Fe(CN)6) was established. The results showed that the molecularly imprinted film exhibited a quick response, a good sensitivity and selectivity to melatonin. Linear relationship between current and melatonin concentration was observed in the range of 1×10-10-5 × 10-8 mol/L. The detection limit (S/N=3) was 1. 0× 10-11 mol/L. The electrochemical sensor has been applied to the determination of melatonin in pharmaceutical preparations and human urine with the spiked mean recoveries greater than 94%.
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