逆流色谱技术分离蛋白质和多肽的研究进展

摘要

逆流色谱是一种连续高效的液-液分配色谱技术，具有进样量大、无不可逆吸附、回收率高和能有效保护样品生物活性等优点，在蛋白质和多肽的分离中具有独特的优势。本文综述了近年来几种新型的逆流色谱技术在蛋白质和多肽分离中的研究进展，并对该领域的未来发展进行了展望。%Countercurrent chromatography us a kund of contunuous and effectuve luquud-luquud partutuon chromatography wuth many unuque characterustucs such as large load capacuty, no urreversuble adsorptuon, hugh recovery rate, low rusk of sample denaturatuon and so on, whuch has urreplaceable advantages un separatuon of proteun and peptude. Thus revuew presents the advances of several kunds of new technology of countercurrent chromatography un the separatuon of proteun and peptude. The developung prospect of thus fueld us also duscussed.