原人参二醇空心金纳米药物载体的制备及体外抗喉癌研究

摘要

原人参二醇(PPD)对多种肿瘤细胞具有抑制作用.但由于水溶性差、利用度低,限制了其在临床上的应用.基于此,本研究以空心金纳米颗粒为PPD的运输载体,合成PPD空心金纳米颗粒,采用高效液相色谱分析PPD空心金纳米药物载体的缓释效应性、MTT法检测对Hep-2细胞增殖的抑制作用、流式细胞术研究对Hep-2细胞凋亡的影响,以考察原人参二醇(PPD)空心金纳米药物载体的体外抗喉癌细胞Hep-2效应.结果表明,PPD空心金纳米药物载体具有缓释效应性,与空白对照组、HAuNs组及PPD组相比,HAuNs-PEG-PPD组的Hep-2细胞生存率下降更显著,细胞凋亡率增加更显著 (p<0.01).PPD空心金纳米载体可显著增强PPD的体外抗喉癌细胞效应,促进肿瘤细胞的凋亡,有望成为PPD新型纳米药物,并应用于喉癌的治疗研究.%Protopanaxadiol (PPD) has inhibitory effects on many tumors and receives much attention. However,it has poor water solubility and low utilization, which limits its clinical application. Considering these issues,in this study,we used hollow gold nanoparticles as transport carriers of PPD and synthesized PPD hollow gold nanoparticles (HAuNs). We conducted a number of experiments to investigate the in vitro anti-laryngeal cancer Hep-2 effect of a PPD HAuNs carrier. High performance liquid chromatography(HPLC) was used to detect the sustained release effect of PPD HAuNs. MTT assay was used to detect the inhibitory effect of PPD HAuNs on the proliferation of Hep-2 cells. Effect of PPD HAuNs on Hep-2 cell apoptosis was investigated by flow cytometry. The results of in vitro release showed that PPD HAuNs had sustained release effect. Compared with blank control group,HAuNs group and PPD group,the survival rate of Hep-2 cells in HAuNs-PEG-PPD group decreased more significantly and the apoptosis rate increased more significantly (p<0.01). PPD HAuNs could significantly enhance anti-laryngeal cancer effect of PPD in vitro and promote the apoptosis of tumor cells. It promotes tumor cell apoptosis, and is expected to be a new PPD drug delivery system, further promoting the application of PPD in clinical anti-laryngeal cancer.