血清微环境对小鼠T细胞衰老的调节作用

摘要

目的:探讨血清微环境对小鼠T细胞衰老的调节作用.方法:分别取年老(12～14月龄)及年轻(1.5～2月龄)小鼠各10只,提取其脾脏淋巴细胞及血清,实验分4组.组Ⅰ为年老鼠T淋巴细胞+10%年轻鼠血清;组Ⅱ为年老鼠T淋巴细胞+10%年老鼠血清;组Ⅲ为年轻鼠T淋巴细胞+10%年轻鼠血清;组Ⅳ为年轻鼠T淋巴细胞+10%年老鼠血清.培养48h后,经流式细胞术研究CD8+CD28+共表达率差异.结果:组Ⅰ和组Ⅱ T细胞表面的CD8+CD28+共表达率分别是(10.84±0.6841)%和(3.18±0.1789)%,组Ⅲ和组Ⅳ T细胞表面的CD8+CD28+共表达分别是(12.5±0.9445)%和(8.36±0.2074)%.各组间对比有统计学差异(P<0.05)结论:血清微环境具有调节小鼠T细胞衰老的作用,年轻鼠血清能使年老鼠的T细胞表面的CD8+CD28+共表达率提高,年老鼠的血清能使年轻鼠的T细胞表面的CD8+CD28+共表达率降低.%Objective To observe the effects of serum microenvironment on CD8 +CD28 +T cells. Methods Mouse lymphocytes were isolated from spleen,these cells from young mouse and old mouse were cultured under the following serum microenvironment. All mouse were divided into four groups: the lymphocytes from old mouse cultured with young serum (group Ⅰ);the lymphocytes from old mouse cultured with old serum (group Ⅱ),the lymphocytes from young mouse cultured with young serum (group Ⅲ), and the lymphocytes from young mouse cultured with old serum (group Ⅳ). The co-expression of CD8+CD28+ on T cells was detected by the flow cytometry. Results In the group of lymphocytes from old mouse cultured with young serum, the co-expression of CD8+CD28+ was (10.84±0.6841)％. In the group of tymphocytes from old mouse cultured with old serum group.The co-expression of CD8+CD28+ was (3.18±0.1789)％. In the group of lymphocytes from young mouse cultured with young serum and cultured with old serum group,the coexpression of CD8+CD28+were (12.5±0.9445)％ and (8.36±0.2074)％, respectively. There were significant statistical differences in these four groups. Conclusion Our study revealed that young serum could improve the co-expression of CD8+CD28+ on T cells. So the senescence of T cells can be regulated by serum.