基质金属蛋白酶-2、14及RECK基因在涎腺多形性腺瘤的初发、复发及恶变中的表达及意义

摘要

目的：研究基质金属蛋白酶-2（matrix metalloproteinase 2，MMP-2）、基质金属蛋白酶-14（matrix metalloproteinase 14，MMP-14）和RECK基因在涎腺多形性腺瘤（pleomorphic adenoma，PA）初发患者、复发患者及进一步恶变患者组中的表达及其意义。方法：研究对象为新疆医科大学第一附属医院颌面肿瘤外科2004年至2014涎腺PA手术标本50例，其中初发36例，恶变7例，复发7例和10例正常腮腺组织（10例均来自腮腺）。通过免疫组织化学方法检测RECK、MMP-2和MMP-14在各组标本中的表达，并采用半定量方法进行计量。对RECK与MMP-2及MMP-14在各组中的表达采用SPSS 21.0软件包对数据进行统计学分析。结果：MMP-2在正常组与复发组差异有统计学意义（P<0.05），正常组与恶变组差异有统计学意义（P<0.05），初发组与复发组差异有统计学意义（P<0.05），初发组与恶变组差异有统计学意义（P<0.05）。RECK与MMP-2和MMP-14在各个组的表达都呈负相关（r=-0.887，P<0.05；r=-0.798，P<0.05）。结论：临床上，在涎腺PA恶变，复发组织同时对RECK与MMP-2的进行检测分析其有重要意义，这对于判别肿瘤侵蚀性和预后指标是极有可能。%Objective To investigate the expression and significance of RECK gene and Matrix Metalloproteinase- 2(MMP- 2),Matrix Metalloproteinase- 14(MMP- 14) in the primary plemorphic adenoma(PA), recurrent PA and malignant PA. Methods Fifty samples including 36 primary PA,7 recurrent PA,7 malignant PA and 10 normal parotid glands as control were examined immunohistocheemically.Among the 10 PA samples,there were all samples originated from parotid gland.RECK gene,MMP- 2 and MMP- 14 were decected immunohistochemically and semi-quantitively in these samples.All date were analyzed through SPSS 21.0 software. Results MMP-2 has significant difference in normal group and recurrent group(P<0.05),the difference was statistically significant in normal group and the malignant group (P<0.05);the difference was statistically significant in primary group and recurrent group(P<0.05);the difference was statistically significant in primary group and the malignant group(P<0.05).The expression of RECK and MMP-2 and MMP-14 were negative correlation,respectively,in normal parotid gland,primaryPA,recurrent PA and maligant PA(r=-0.887,P<0.05;r=0.798,P<0.05). Conclusion Clinically,in the tissue of the salivary glands,PA will relapse.When examined both RECK and MMP- 2 have significant meaning.Through this examination,tumor aggressiveness and prognosis can be indicated.