丹参对大鼠皮肤创伤修复及血管内皮生长因子表达的影响

摘要

Objective To observe the effects of Salvia Miltiorrhiza on repair area of skin wound and expression level of vascular endothelial growth factor(VEGF)in skin wound repair model of rat.Methods 36 SD(Sprague-dawley)rats were randomly divided into control group and Salvia Miltiorrhiza group,18 rats in each group,all rats were subjected to the full-thickness skin excision.The Salvia Miltiorrhiza group was treated with Salvia Miltiorrhiza injection to repair the wound,and the control group was treated with the same dose of physiological saline.The wound unrepaired area was measurement of all the animal models,and the pathologicchange and the expression change of VEGF were observed by HE staining and immunohistochemistry.Results Determination of unrepaired area of skin wound showed that after injury 1d,3d,7d control group and Salvia Miltiorrhiza group of rat skin wounds unrepaired area was gradually reduced,but it was lower than the control group in the Salvia Miltiorrhiza group after injury 3d,7d.In the Salvia Miltiorrhiza group the erythrocyte sedimentation in vessels was lighter than the control group and the number of new capillaries was more than the control group by HE staining after 3d of injury.Immunohistochemical staining showed that VEGF was located in animal skin epidermal cells,sebaceous gland,hair follicle,neutrophils,macrophages,fibroblasts,but fibroblast was in the majority.After injury 1d,3d,the VEGF expression intensity in the Salvia Miltiorrhiza group was higher than the control group(P<0.05).Conclusion Salvia Miltiorrhiza can promote the repair of traumatic skin in rats by increasing the expression level of VEGF.%目的:观察丹参对大鼠皮肤创伤修复效果及血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平的影响.方法:36只SD(Sprague-dawley)大鼠随机分为对照组和丹参组,每组18只,采用全层皮肤切除制备创伤修复动物模型,丹参组采用丹参注射液修复创面,对照组用相同剂量的生理盐水.造模后1d、3d、7d测量伤口未愈面积,并通过HE染色和免疫组织化学染色方法观察损伤区域不同时间的组织病理学改变和VEGF的表达变化.结果:大鼠皮肤创伤未愈合面积测定结果显示损伤后1d、3d、7d对照组和丹参组大鼠创伤皮肤未愈合面积均逐渐缩小,3d、7d后丹参组未愈合面积均小于对照组(P<0.05);HE染色可观察到损伤后3d丹参组小血管内红细胞淤积情况较对照组轻,新生毛细血管数量较对照组多;免疫组化染色结果显示VEGF在大鼠皮肤表皮细胞、皮脂腺上皮细胞、毛囊、中性粒细胞、巨噬细胞、成纤维细胞中有表达,但以成纤维细胞的表达为主.损伤后1d、3d,丹参组VEGF表达强度高于对照组(P<0.05).结论:丹参可通过增加血管内皮生长因子的表达水平促进大鼠创伤皮肤的修复.