Quantitative analysis of nicotinic acid,nicotinamide and 3-cyanopyridine in industrial effluent by high performance liquid chromatography

摘要

A rapid and reliable high performance liquid chromatographic(HPLC) method for the simultaneous determination of heterocyelic compounds,namely nicotinic acid,nicotinamide,and 3-cyanopyridine,in industrial effluent is described.A Φ4.6 mmx 150 mm,5 μm C-18 reversed phase stationery phase,and a methanol-acetonitrile-water tertiary mobile phase (20:20:60v/v) were used for separation.The detection wavelength of a diode array (DAD) was set at 216 nm with a bandwidth of 16 nm.Phenol was used as an internal standard.The regression equations revealed a linear relationship between the concentration of the analytes injected and the peak area detected by DAD.The limits of detection (S/N = 3) ranged from 0.70 to 1.18 mg L-1,the recoveries ranged from 87% to 102% and the precision expressed as % RSD intra-day and inter-day varied from 0.9 to 3.9 and 1.2 to 5.6,respectively.This method is rapid,sensitive and suitable for the monitoring of nicotinic acid,nicotinamide,and 3-cyanopyridine in effluent of related pharmaceutical manufacturing plants.