胆固醇酯转运蛋白 C ﹥T/In9基因多态性与广西人群2型糖尿病亚临床动脉粥样硬化的相关性

摘要

Objective To investigate the distribution characteristics of cholesterol ester transfer protein(CETP)C ﹥T/ In9(rs289714)gene polymorphism of the population in Guangxi and its correlation with subclinical atherosclerosis(SA)in patients with type 2 diabetes mellitus( T2DM). Methods We enrolled 83 T2DM patients who were admitted into the Department of Endocrinology and Metabolism of the First Affiliated Hospital of Guangxi Medical University from December 2012 to June 2014 as T2DM group,and the 83 patients all accorded with the criteria of diabetes diagnosis and typing. We also enrolled 68 healthy people who received physical examination in the hospital in the same period as control group. PCR-RFLP was employed to examine the CETP C ﹥ T/ In9 gene polymorphism. Results Control group and T2DM group were not significantly different in the distribution of C ﹥ T/ In9 genotypes and allele frequency(χ2 = 0. 943,0. 004;P ﹥ 0. 05). In T2DM group,the proportions of TT,CC and CT genotypes were 67. 4% ,10. 9% and 21. 7% for SA subgroup and 45. 9% ,2. 7% and 51. 4% for non - SA subgroup,with significant differences between the two subgroups( χ2 = 8. 367,P = 0. 014). In T2DM group,SA subgroup and non - SA subgroup were not significantly different in T and C allele frequency( χ2 = 0. 972,P = 0. 324). Multivariate Logistic regression analysis showed that CETP C ﹥ T/ In9 genotype and age were influencing factors for T2DM patients with SA in Guangxi Province 〔 OR ( 95% CI ) = 0. 560 ( 0. 317,0. 989 );1. 126 ( 1. 054,1. 203 ), P ﹤ 0. 05 〕. Conclusion For the population of Guangxi,CETP C ﹥ T/ In9 gene polymorphism could not be considered related with glycometabolism;T2DM patients with SA and those without SA are different in CETP C ﹥ T/ In9 genotype distribution. CETP C﹥ T/ In9 gene polymorphism may be related with SA in T2DM patients,and TT genotype may be the damaging genotype.%目的：探讨广西地区人群胆固醇酯转运蛋白（CETP）C ﹥ T/ In9（rs289714）基因多态性分布特点，及其与糖代谢和2型糖尿病（T2DM）亚临床动脉粥样硬化（SA）的相关性。方法选择2012年12月—2014年6月于广西医科大学第一附属医院内分泌代谢病科住院的 T2DM 患者83例为 T2DM 组，均符合1999年 WHO 推荐的糖尿病诊断及分型标准；选择该院体检中心同期体检健康者68例为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测两组 CETP C ﹥ T/ In9基因多态性。结果对照组和 T2DM 组 C ﹥ T/ In9基因型分布及等位基因频率比较，差异均无统计学意义（χ2＝0.943、0.004，P ﹥0.05）。T2DM 组 SA 亚组 TT、CC、CT 基因型分布分别为67.4％、10.9％、21.7％，非 SA 亚组分别为45.9％、2.7％、51.4％，差异有统计学意义（χ2＝8.367，P ＝0.014）。T2DM 组 SA 亚组与非 SA 亚组 T、C 等位基因频率比较，差异无统计学意义（χ2＝0.972，P ＝0.324）。多因素 Logistic回归分析显示，CETP C ﹥ T/ In9基因型和年龄是广西人群 T2DM 发生 SA 的影响因素〔OR（95％ CI）＝0.560（0.317，0.989）、1.126（1.054，1.203），P ﹤0.05〕。结论在广西人群中，暂不能认为 CETP C ﹥ T/ In9基因多态性与糖代谢有关；CETP C ﹥ T/ In9基因型在 T2DM SA 和非 SA 人群中分布不同，CETP C ﹥ T/ In9基因多态性可能与 T2DM SA 相关，基因型 TT 可能是损害性基因型。