不同方法学检测乙型肝炎血清标志物结果的评价分析

摘要

目的：探讨不同方法学检测乙型肝炎血清标志物结果的灵敏性。方法：2012年1月-2014年1月收治乙型肝炎患者124例，分别采用化学发光法(CLIA)、酶联免疫吸附试验(ELISA)、时间分辨免疫荧光法(TRFIA)对从患者身上获得的乙型肝炎标志物进行检测。对比分析以上3种检测方法的阳性检测率。结果：CLIA、ELISA、TRFIA 3种检测方法中，CLIA的灵敏度最高。并且CLIA、ELISA、TRFIA 3种检测方法的阳性检出率分别为92.5%、50.0%、36.3%；3组比较差异具有统计学意义(P＜0.05)。结论：不同方法在检测乙型肝炎时的灵敏性不同，通过比较CLIA、ELISA及TRFIA 3种方法的检测结果，发现CLIA的阳性检出率最高，并且也最灵敏，因而该方法值得临床推广应用。%Objective:To explore the sensitivity of different methodologies for detection of serum markers of hepatitis B results. Methods:124 patients with hepatitis B were selected from January 2012 to January 2014.Hepatitis B markers were detected respectively by chemiluminescence(CLIA),enzyme linked immunosorbent assay(ELISA),time-resolved fluorescence immunoassay(TRFIA).We compared the positive detection rate of the three methods.Results:In CLIA,ELISA,TRFIA detection, CLIA sensitivity was the highest.The positive rate of CLIA,ELISA,TRFIA was respectively 92.5%,50%,36.3%,and the difference was statistically significant(P<0.05).Conclusion:In the detection of hepatitis B,the sensitivity of different methods was different, through the comparison of the CLIA,ELISA,TRFIA detection,CLIA positive rate was the highest,and the sensitivity was the highest,so this method was worthy of clinical application.