溶血现象对临床生化检验项目影响的观察

摘要

Objective:To investigate the influence of hemolysis phenomenon on clinical biochemistry tests.Methods:300 healthy subjects were selected from January 2014 to August 2014.They were taken 5 mL blood averaged into 2 tubes.One teams with conventional treatment and then inspected;another were inspected after artificial hemolytic,unified the standard of quality control, then screen abnormality index by the physician with abnormal double blind method.Results:The levels of ALT,AST,LDH, K+, CHOL in hemolysis specimens were higher than normal specimens,while the ALP level lower than the normal specimens;the abnormal rate of the normal group was 7.33% lower than that of the hemolytic group which was 85%,and the normal rate was 92.67% higher than of the hemolytic group which was 15%,and the difference was statistically significant(P<0.05);while,the level of TG,BUN,Cr,GLU and UA had no statistically different between the normal specimens and the hemolytic samples(P>0.05). Conclusion:Hemolytic can influence a number of blood biochemical index such as ALT,AST,LDH,K + and CHOL etc;regular check without artificial hemolysis still exist inspection errors;so we should improve the level of quality control in blood biochemical inspection.%目的：探讨溶血现象对临床生化检验项目的影响。方法：2014年1月-2014年8月收治健康体检者300例，采血5 mL平均分配至2支试管中，1支常规处理送检，1支人工溶血送检，统一质控标准，由检验科医师采用双盲法筛查指标异常者。结果：溶血标本 ALT、AST、LDH、K＋、CHOL水平高于正常标本，ALP水平低于正常标本，正常组异常率7.33%低于溶血组的85.00%，正常率92.67%高于溶血组的15.00%，差异具有统计学意义(P＜0.05)；正常标本与溶血标本TG、BUN、Cr、GLU、UA水平差异无统计学意义(P＞0.05)。结论：溶血可影响ALT、AST、LDH、K＋、CHOL等多项血液生化指标结果；未进行人工溶血，常规检查，仍存在检验差错；应提高血液生化检验质控水平。