支气管肺泡灌洗术在小鼠肺纤维化模型中的应用

摘要

Objective:To explore the feasibility of improving the recovery rate of bronchoalveolar lavage fluid(BALF)in mice with pulmonary fibrosis by improving the experimental method.Methods:Male BALB/C mice were selected.36 male mice from 6 to 8 week were divided into the 3 groups.After 28 days of pulmonary fibrosis,the bronchoalveolar lavage was performed 4 times,0.8 mL/times,and repeated 4 times.Macrophages and lymphocytes were the main inflammatory cells in the lavage fluid.Results:Through this method each mouse can be collected BALF about 2.6~2.8 mL/3.2 mL.There was no statistical difference between groups,and there was no significant difference between groups(P>0.05).The recovery rate was about 81.25%~87.5%,and the cell count could meet the experimental requirements.Conclusion:The recovery rate of alveolar lavage fluid obtained from this method can meet the need of medical scientific research,and it has the characteristics of strong operability and practicability.%目的：通过改进实验方法来提高肺纤维化小鼠支气管肺泡灌洗液(BALF)回收率的可行性。方法：选取雄性 BALB/C 小鼠，6～8周龄共36只分成3组。肺纤维化造模28 d 后，对每只小鼠进行支气管肺泡灌洗术4次，0.8 mL/次,反复抽注4次，灌洗液中的炎性细胞以巨噬细胞和淋巴细胞为主。结果：通过本文方法，每只小鼠可收集到的BALF 2.6～2.8 mL/3.2 mL，组间差异无统计学意义(P＞0.05),回收率81.25%～87.5%,其中细胞计数达到实验要求。结论：本方法得到的肺泡灌洗液的回收率可满足医学科研的需要，具有可操作性强且实用的特点。