阿司匹林联合曲妥珠单抗对HER-2阳性乳腺癌细胞增殖和凋亡的影响

摘要

Objective To investigate the effects of aspirin combined with trastuzumab on proliferation and apoptosis of HER-2-positive breast cancer cell line SKBR-3.Methods The SKBR-3 cells were divided into four groups:control group,aspirin group (5 mmol/L),trastuzumab group (30 μg/ml) and combination group (5 mmol/L aspirin+30 μg/ml trastuzumab).MTT assay and flow cytometry were used to observe the effects of different drug treatment on cell proliferation and apoptosis.Results MTT assays showed that the relative proliferation rate of SKBR-3 cells was (79.6±2.61) ％ in aspirin group,(48.2±3.35) ％ in trastuzumab group and (21.5± 1.66)％ in combination group.Compared with aspirin group or trastuzumab group,the cell proliferation of combination group was significantly inhibited (P＜0.05).Two drugs combined effect index was 1.0 of a superimposition effect.The apoptosis assays showed that the relative apoptotic rate of cells was (27.3±3.47)％ in aspirin group,(35.3±t2.80)％ in trastuzumab group and (56.2 ±3.95)％ in combination group.Compared with aspirin group or trastuzumab group,the cell apoptosis of combination group was significantly increased (P＜0.05).Conclusion Aspirin combined with trastuzumab can effectively inhibit the growth of HER-2-positive breast cancer cells,which may be a new model for the clinical treatment of HER-2-positive breast cancer.%目的 探讨阿司匹林联合曲妥珠单抗对表皮生长因子受体2 (HER-2)阳性乳腺癌SKBR-3细胞增殖和凋亡的影响.方法 将SKBR-3细胞分为对照组、阿司匹林组(5 mmol/L)、曲妥珠单抗组(30 μg/ml)和联合组(5 mmol/L阿司匹林+30 μg/ml曲妥珠单抗),采用MTT法、流式细胞术分别观察不同药物处理对SKBR-3细胞增殖和凋亡的影响.结果 阿司匹林组SKBR-3细胞增殖率为(79.6±2.61)％,曲妥珠单抗组为(48.2±3.35)％,联合组为(21.5±1.66)％,组间差异有统计学意义(P＜0.05).阿司匹林、曲妥珠单抗联合作用指数(CI)为1.0,属于叠加作用.阿司匹林组SKBR-3细胞凋亡率为(27.3±3.47)％,曲妥珠单抗组为(35.3±2.80)％,联合组为(56.2±3.95)％,组间差异有统计学意义(P＜0.05).结论 阿司匹林联合曲妥珠单抗能有效抑制HER-2阳性乳腺癌细胞的增殖,两药联合有可能成为HER-2阳性乳腺癌的临床治疗新模式.