高效液相色谱法测定氯霉素滴眼液中的二醇物

摘要

目的：建立氯霉素滴眼液中二醇物的测定方法。方法：采用高效液相色谱法，以 C18为固定相，甲醇－ 0 4％磷酸溶液（ 55∶ 45）为流动相，检测波长为 254nm。结果：平均回收率为 98 5％， RSD=0 86％。结论：本法操作简便，结果准确可靠，适合氯霉素滴眼液中二醇物的测定。%OBJECTIVE： To establish a method for detecting glycols in chloramphenicol eye drops METHODS： HPLC method was used with C18 column as stationary phase and methanol－ 0 4％ phosphoric acid（ 55∶ 45） as mobile phase The detection wavelength was 245nm RESULTS： The average recovery was 98 5％ （ RSD=0 86％ ）  CONCLUSION： The method is simple， reliable and suitable for detecting glycols in chloramphenicol eye drops