酶联免疫吸附检测法检测全血中他克莫司浓度及其应用

摘要

目的：探讨酶联免疫吸附检测法(ELISA)检测全血中FK506浓度的准确性及其在器官移植术后抗排斥治疗中的应用。方法：采用酶联免疫吸附检测法，对5例肝移植术后患者不同时期全血中FK506谷值浓度进行监测，并结合临床调整剂量，分析该法的准确性及其对临床FK506应用的指导作用。结果：在305个血样的131次检测中，质控血样检测结果全部在控制范围内，高、低浓度两个质控的RSD值分别为9.09％和11.90％，监测下的FK506血药浓度控制与文献报道一致。结论：酶联免疫吸附检测法灵敏度高、特异性强，是一种较理想的FK506血药浓度常规检测方法，适宜在医院内开展。%OBJECTIVE:To discuss the accuracy of detecting tacrolimus(FK506) concentration in whole blood by ELISA and its application in organ transplantation.METHODS:The ELISA was used to detect FK506 valley point concentrations in 5 liver transplanted recipients at different postoperative periods .Based on the results combined with clinical findings, the dosages were adjusted.The accuracy of the method and guidance action in FK506 clinical application were described.RESULTS: In 131 detections of 350 samples, the results of quality control samples were all in the control range .The detecting RSDs of high and low concentration quality control samples were 9.09% and 11.90%, respectively.The control range of FK506 concentrations in whole blood were similar to that reported in literature .CONCLUSION :ELISA is a sensitive and specific method for routine FK506 concentration monitoring in whole blood,which is suitable for application in hospital.