RP-HPLC法测定奇力咳感康口服液中黄芩苷的含量

摘要

OBJECTIVE：To establish a method for determination of baicalin in QiLiKeGanKang oral liquid.METHODS：The RP-HPLC method was employed taking Nova-pak C18 column as fixed phase and methanol-water-phosphoric acid(45∶ 55∶ 0.2)as mobile phase.Detection wavelength was 280 nm.RESULTS：The detection ranges were 0.179μ g～1.434μ g.The average recovery of baicalin was 101.35％ (RSD=2.40％，n=5).CONCLUSION：This HPLC method is convenient，rapid，accurate and could be used for quality control in the production of QiLiKeGanKang oral liquid.%目的：建立奇力咳感康口服液中黄芩苷的含量测定方法。方法：采用RP-HPLC法，固定相是Nova-pakC18柱，流动相为甲醇-水-磷酸(45∶55∶0.2)，在280nm波长处检测。结果：黄芩苷的线性范围为0.179μg～1.43μg(r=0.9998)，加样回收率为101.35％，RSD为2.40％(n=5)。结论：本法简便、快速、准确，可作为控制奇力咳感康口服液质量的方法。