HPLC法测定江西不同产地蔓荆子中蔓荆子黄素的含量

摘要

OBJECTIVE: To establish a method for the content determination of vitexicarpin in Vitex trifolia from different producing areas of Jiangxi province. METHODS: HPLC method was adopted. The determination was performed on Alltima HP C18 (250 mm ×4.6 mm,5 μm) column with the mobile phase consisted of acetonitrile-50 mmol·L-1 potassium dihydrogen phosphate solution (pH value adjusted to 3.0 with phosphoric acid, 50:50), at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 254 nm, and the column temperature was room temperature. The injection volume was 10 μL. RESULTS: The linear range was 0.2~1.2 μg(r=0.999 4) with an average recovery of 99.96% (RSD= 1.42%, n=9).Vitexicarpin contents of V. trifolia from different areas differed greatly. CONCLUSION: The method is simplp and rapid with good linear relationsgip, which can be used for the content determination of vitexicarpin in V. trifolia.%目的:建立测定江西不同产地蔓荆子中蔓荆子黄素含量的方法.方法:采用高效液相色谱法.色谱柱为Alltima HPC18(250mm×4.6 mm,5 μm),流动相为乙腈-50 mol·L-1磷酸二氢钾溶液(磷酸调pH值为3.0)=50:50,流速为1.0 mL·min-1,检测波长为254nm,柱温为室温,进样量为10 μL.结果:蔓荆子黄素的进样量在0.2～1.2 μg范围内与峰面积积分值呈良好的线性关系(r=0.999 4);平均加样回收率为99.96％,RSD=1.42％(n=9).江西不同产地蔓荆子中蔓荆子黄素含量差异较大.结论:本方法简便、快速、线性关系良好,可用于蔓荆子中蔓荆子黄素的含量测定.