心脑肾康对脑缺血再灌注模型大鼠的保护作用研究

摘要

OBJECTIVE: To study the protective effect of Xinnao shenkang on cerebral ischemia model rats. METHODS: The cerebral ischemia-reperfusion (MCAO) model was induced by thread occlusion method. Those model rats were divided into 6 groups, i.e. sham operation group (constant volume of normal saline), model group (constant volume of normal saline), Xingling group (2 g·kg-1) and Xinnao shenkang high-dose, medium-dose and low-dose groups (32,16,8 g·kg-1). The brain tissue biochemical index and platelet aggregation risistance index were determined, and range of cerebral infarction was observed by TTC staining. RESULTS: Compared with model group, the range of cerebral infarction decreased significantly in Xinnao shenkang high-dose, medium-dose and low-dose groups, and the levels of NOS, ROS, MDA and LA also decreased significantly. SOD and ATP levels were increased significantly, and the platelet aggregation resistance index also increased significantly (P<0.01). CONCLUSION: Xinnao shenkang on cerebral ischemia reperfusion model rats have a protective effect.%目的:研究心脑肾康对脑缺血再灌注(MCAO)模型大鼠的保护作用.方法:以线栓法复制大鼠MCAO模型.实验分为6组,即假阻断(等容生理盐水)、模型(等容生理盐水)、杏灵(2 g·kg-1)与心脑肾康高、中、低剂量(32、16、8 g·kg-1)组.测定大鼠大脑皮层生化指标与血小板聚集阻率,TTC染色观察脑梗死病变范围.结果:与模型组比较,心脑肾康高、中、低剂量组大鼠脑梗死范围显著减小,一氧化氮合酶、活性氧、丙二醛、乳酸水平显著降低,超氧化物歧化酶、三磷酸腺苷水平显著升高,血小板聚集阻率显著升高(P＜0.01).结论:心脑肾康对脑缺血再灌注模型大鼠有一定保护作用.