定位超声辐照微泡联合红花黄色素对急性心肌梗死模型大鼠心功能与左室重塑的影响

摘要

目的:研究定位超声辐照微泡联合红花黄色素对急性心肌梗死模型大鼠心功能与左室重塑的影响.方法:以结扎冠脉前降支复制大鼠急性心肌梗死模型.实验分为假手术(等容生理盐水,腹腔注射)、模型(等容生理盐水,腹腔注射)、定位超声辐照微泡(辐照10s,停10s,共2 min)、红花黄色素(40 mg/kg,腹腔注射)、定位超声辐照微泡+红花黄色素(辐照10s,停10s,共2 min+40mg/kg,腹腔注射)组.术后立即给药,每天1次,连续28 d；术后3d开始辐照,每周1次,连续28 d.彩色多普勒超声诊断仪检测大鼠心功能,计算左室质量指数,RT-PCR法检测白细胞介素(IL)-1β mRNA水平表达.结果:与模型组比较,定位超声辐照微泡、红花黄色素、定位超声辐照微泡+红花黄色素组大鼠心功能显著改善,左室质量指数、IL-1β mRNA的表达显著下降(P＜0.05),其中定位超声辐照微泡+红花黄色素组上述各项指标改善最显著.结论:定位超声辐照微泡促血管再生治疗与红花黄色素均可以改善心功能,抑制炎性细胞因子IL-1β mRNA的表达,降低左室质量指数,改善左室重塑,二者联合治疗具有协同作用.%OBJECTIVE:To investigate the effect of targeted ultrasound irradiation microbubble (TUIM) combined with carthamin yellow on cardiac function and left ventricular remolding in rats with acute myocardial infarction.METHODS:Rat model with acute myocardial infarction was established using ligation of left anterior descending branch.Model rats were divided into sham operation group (constant volume of normal saline,intraperitoneal injection),model group (constant volume of normal saline,intraperitoneal injection),UTIM group (intermitant irradiation 10 s,10 s interval,2 min in total),CY group(40 mg/kg,intraperitoneal injection),UTIM+CY group (intermitant irradiation 10 s,2 min in total+40 mg/kg,intraperitoneal injection).The rats were given medicines after operation,once a day for consecutive 28 days.The rats were irradiated 3 days after operation,once a week for consecutive 28 days.The cardiac functions were detected by Color Doppler ultrasound.Left ventricular weight index were calculated.The mRNA expression of IL-13 was detected by RT-PCR.RESULTS:Compared with model group,cardiac functions of UTIM group,CY group and UTIM +CY group were improved significantly,and left ventricular weight index and mRNA expression of IL-13 were decreased significantly(P＜0.05); the improvement of above index in UTIM +CY group were more significant than in other 2 groups.CONCLUSIONS:Both UTIM revascularization therapy and carthamin yellow can improve cardiac function,inhibit the mRNA expression of inflammatory cytokines IL-1β,reduce left ventricular mass index and improve left ventricular remodeling.Combination of both therapies has a synergistic effect.