氟西汀对卒中后抑郁模型大鼠脑组织中5-HT、NE及NGF表达的影响

摘要

OBJECTIVE:To study the influence of fluoxetine on the levels of 5-hydroxytryptamine (5-HT),norepinephrine (NE) and nerve growth factor (NGF) in brain tissue of rats with post-stroke depression (PSD).METHODS:Rats were randomized into sham-operated group,depression model group,stroke model group,PSD model group and fluoxetine group (10 mg/kg) with 12 rats in each group.The depression model was induced by chronic unpredictable mild stress (CUMS) combined with depression model,and stroke model was induced by middle cerebral artery occlusion (MCAO).Depression model was established in PSD model group and fluoxetine group after inducing stroke model.They were given relevant medicine intraperitoneally for consecutive 21 days,and ELISA and immunohistochemical SABC were used to detect the expression of 5-HT,NE and NGF in brain tissue.RESULTS:Compared with sham-operated group,the expressions of 5-HT,NE and NGF in brain tissue were significantly lowered in depression model group and PSD model group (P＜0.01) ; compared with depression model group and PSD model group,the expressions of 5-HT,NE and NGF in brain tissue were significantly increased in fluoxetine group (P＜0.01),and there was no significant difference between fluoxetine group and sham-operated group (P＞0.05).CONCLUSIONS:Fluoxetine could increase 5-HT,NE and NGF levels in the brain tissue of PSD rats significantly.%目的:研究氟西汀对卒中后抑郁(PSD)模型大鼠脑组织中5-羟色胺(5-HT)、去甲肾上腺素(NE)及神经生长因子(NGF)表达的影响.方法:将大鼠随机分为假手术组、抑郁模型组、卒中模型组、PSD模型组和氟西汀(10 mg/kg)组,每组12只.采用慢性温和不可预知应激(CUMS)结合孤养建立抑郁模型,大脑中动脉缺血(MCAO)术建立卒中模型,PSD模型组和氟西汀组大鼠先建立卒中模型后建立抑郁模型,腹腔注射相应药物进行干预,连续给药21d后,用酶联免疫法和免疫组化SABC法检测给药后各组大鼠脑组织中5-HT、NE、NGF的表达.结果:与假手术组比较,抑郁模型组和PSD模型组大鼠脑组织中5-HT、NE、NGF表达均明显降低(P＜0.01)；与抑郁模型组和PSD模型组比较,氟西汀组大鼠脑组织中5-HT、NE、NGF表达均明显升高(P＜0.01),且氟西汀组与假手术组比较无显著性差异(P＞0.05).结论:氟西汀能显著提高PSD模型大鼠脑组织中5-HT、NE、NGF水平.